高效液相色谱法快速测定银杏叶提取物中的萜类内酯

摘要

A new rapid analytical method was developed for the quantification of terpene lactones (bilobalide(BB) and ginkgolide A,B,C,J) in extract of Ginkgo biloba L. (EGb) using a liquid-liquid solvent extraction procedure followed by high performance liquid chromatography. EGb was dissolved in 30% ethanol and extracted with ether. After evaporation, the residue was then determinated by HPLC on a C18 column with methanol-water-orthophosphoric acid (25∶75∶0.1,V/V) as eluent and refractive index (RI) detection. Results showed that the excellent sample clean-up procedure is more simple and specific, and saves more time (less than 20 min) than any other methods that have been reported, and also leads to high recoveries (>99.0%) and low RSDs (<2.0%).The reproducible method is regarded to be very useful for evaluating the quality of extract of Ginkgo biloba L..%建立了液-液萃取后高效液相色谱法(HPLC)测定银杏叶提取物(EGb)中萜类内酯含量的快速方法.EGb样品溶于体积分数为30%的乙醇溶液后用乙醚萃取,有机相浓缩后的残留物以HPLC分析,组分的分离采用C18柱,以甲醇-水-磷酸(体积比为25:75:0.1)为流动相,示差检测器检测.结果表明,样品净化程序较已报道的方法更简便,选择性强、快速(少于20 min).该方法回收率大于99.0%、相对标准偏差小于2.0%、重现性好,可有效地用于银杏叶提取物的产品质量评价.