A method was developed to determine chromium speciation simultaneously in cosmetics using reversed-phase ion-pair chromatography ( RP-IPC) with inductively coupled plasma mass spectrometry (ICP-MS). After the extraction with disodium ethylenediaminetetraace-tate( EDTA) in a water bath, the sample was separated on an XDB C18 column with the mobile phase of 5% (v/v) methanol-2. 0 mmol/L tetrabutylammonium (TBA) (Ph6.0), the flow rate of 1.0 Ml/min and the injection volume of 100 Μl. The collision cell technology was applied to eliminate the mass interferences of 40Ar12C+and 35Cl16O1H+in the analysis of 52Cr+. The separation was achieved within 5 min. The recoveries of Cr( M ) and Cr( VI) ranged from 82. 7% to 107.2% with the relative standard deviations (RSDs) less than 5.62% (n =6) with the spiked amounts of 0. 01 -0. 50 μg in different kinds of cosmetic samples. The developed method has the advantages of simplicity, sensitivity and good reproducibility, and can be used for the simultaneous determination of Cr( III ) and Cr( VI) in cosmetics.%建立了反相离子对色谱(RP-IPC)与电感耦合等离子体质谱(ICP-MS)联用技术快速分离测定化妆品中Cr(Ⅲ)和Cr(Ⅵ)的方法.样品经乙二胺四乙酸钠盐(EDTA)50℃水浴中提取后,采用XDB-C18色谱柱分离,以5％ (v/v)甲醇-2.0 mmol/L四正丁基铵盐(TBA)水溶液(pH 6.0)作为流动相,流速为1.0 mL/min,进样量为100μL.采用碰撞池技术消除ICP-MS测定时40Ar12C+、35Cl16O1H+对52Cr+的光谱学干扰.Cr(Ⅲ)和Cr(Ⅵ)的分离过程在5 min内完成.样品中加标量为0.01 ～0.50 μg时,其回收率为82.7％ ～ 107.2％,相对标准偏差(RSD)小于5.62％.该方法操作简便、灵敏度高、重现性好,适合于化妆品中Cr(Ⅲ)和Cr(Ⅵ)的同时分析.
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