白藜芦醇对粥样硬化模型兔血脂及 ADRP 表达的影响

摘要

目的：探讨白藜芦醇对动脉粥样硬化（AS）模型兔血脂及脂肪分化相关蛋白（ADRP）表达的影响。方法：将新西兰兔36只随机分为4组（每组9只）：正常对照组、AS 模型组、白藜芦醇低剂量组和白藜芦醇高剂量组。治疗给药15周，检测各组治疗前后血清脂蛋白的表达水平，主动脉组织脂肪细胞中 ADRP 表达，兔主动脉组织脂肪细胞 ADRP mRNA 的表达，并进行统计学比较。结果：15周末与 AS 模型组相比，白藜芦醇低、高剂量组血清总胆固醇[TC，（18.25±4.35）mmol／L 比（12.79±2.45）mmol／L、（10.35±2.80）mmol／L]，甘油三酯[TG，（3.15±0.94）mmol／L 比（2.63±0.75）mmol／L、（1.18±0.64）mmol／L] 及 低 密 度 脂 蛋 白 胆 固 醇[LDL-C，（15.18±3.65）mmol／L 比（8.35±1.15）mmol／L、（7.18±1.75）mmol／L]含量明显下降，高密度脂蛋白胆固醇[HDL-C，（1.02±0.36）mmol／L 比（3.22±1.02）mmol／L、（2.72±0.82）mmol／L]明显升高（P ＜0.05或 P ＜0.01），白藜芦醇高剂量组甘油三酯[TG，（3.15±0.94）mmol／L 比（1.18±0.64）mmol／L]水平显著降低（P ＜0.01）；白藜芦醇低、高剂量组主动脉组织脂肪细胞中 ADRP 组化染色[（0.43±0.05）比（0.35±0.04）、（0.38±0.15）]及 ADRPmRNA 的表达[（2.47±0.94）比（1.30±0.49）、（1.83±0.61）]均明显减弱（P 均＜0.01）。结论：白藜芦醇能抑制动脉粥样硬化兔主动脉组织脂肪细胞中 ADRP 及其 mRNA 的过度表达，降低血脂，有利于延缓动脉粥样硬化进程。%Objective:To explore the influence of resveratrol on blood lipid level and expression of adipose differenti-ation related protein (ADRP)in atherosclerosis (AS)model rabbits.Methods:A total of 36 New Zealand rabbits were randomly and equally divided into normal control group,AS model group,low dose resveratrol group (low dose group)and high dose resveratrol group (high dose group).Rabbits received 15-week administration,expression level of serum lipoprotein,ADRP and ADRPmRNA expressions in adipocytes of rabbit aortic tissue were measured and compared among all groups before and after treatment.Results:Compared with AS model group at the end of 15th week,there were significant reductions in serum concentrations of total cholesterol [TC,(18.25±4.35)mmol/L vs.(12.79±2.45)mmol/L,(10.35±2.80)mmol/L],triglyceride [TG, (3.15±0.94)mmol/L vs. (2.63± 0.75)mmol/L、(1.18±0.64)mmol/L]and low density lipoprotein cholesterol [LDL-C,(15.18±3.65)mmol/L vs.(8.35±1.15)mmol/L,(7.18±1.75)mmol/L],and significant rise in level of high density lipoprotein choles-terol [HDL-C,(1.02±0.36)mmol/L vs.(3.22±1.02)mmol/L,(2.72±0.82)mmol/L]in low dose group and high dose group (P <0.05 or <0.01),and significant reduction in level of triglyceride [TG,(3.15±0.94)mmol/L vs.(1.18±0.64)mmol/L]in high dose group,P <0.01;ADRP histochemical staining [(0.43±0.05)vs.(0.35± 0.04),(0.38±0.15)]and ADRPmRNA expression [(2.47±0.94)vs.(1.30±0.49),(1.83±0.61)]significantly weakened in adipocytes of aortic tissue in low dose group and high dose group,P <0.01 all. Conclusion:Resveratrol can inhibit the overexpression of ADRP and its mRNA in adipocytes of aortic tissue in AS rabbits,reduce blood lipid levels,and help to delay the AS progression.