乙酰胆碱对人表皮细胞冷存的影响

摘要

Objective: To investigate the effect of acetylcholine (Ach) on the preservation of human keratinocytes. Method: Use tissue engineering technology to separate and culture human keratinocyte in vitro and observe them using MTT method and histological and electron microscopic methods. Result: Different concentration of Ach can preserve more viability of human keratinocytes. The best concentration is 10－ 8 to 10－ 10 mol/L. Conclusion: Ach can preserve more viability of human kerationcytes during preservation.%目的：探讨乙酰胆碱（Ach）对人表皮细胞冷存活力的影响。方法：应用人表皮细胞的分离、培养技术，并进行MTT检测、病理切片以及电镜检测等手段进行观察。结果：不同浓度的Ach对冷存的表皮细胞均有一定的保存活力作用，其中以10－8～10－10mol/L的浓度为最佳。结论：认为Ach对冷存中的人表皮细胞有一定的保护作用，它可提高表皮细胞的保存活力。