原位吸附浸没式膜生物反应器催化制备茚二醇

摘要

在浸没式膜反应器（ IMB）中利用恶臭假单胞菌ATCC 55687催化茚制备顺式茚二醇。采用25根膜丝制作的膜组件，在茚为3 g／L时，经过24 h培养，IMB中顺式茚二醇产量达到悬浮细胞反应器（ SCB）的4倍多；进一步，在培养开始阶段加入10 g／L sp 207树脂进行原位吸附，IMB中顺式茚二醇产量最高达709 mg／L，为SCB产量的660％，容积产率也从SCB中的6 mg／（ L·h）提高到30 mg／（ L·h）。在原位吸附IMB中，中空纤维膜既可作为固定化细胞载体，同时又可作为第二相吸附不溶性底物，降低底物和产物抑制，兼有固定化反应器和两相反应器的优点。%An immersed membrane bioreactor( IMB) with hollow fiber was fabricated for bioconversion of indene to cis-indandiol by Pseudomonas putida ATCC 55687�Compared with suspension culture bioreactor ( SCB) ,an increase of more than four-fold of cis-indandiol at 3 g/L indene concentration was achieved for 24 h in the IMB with a module of 25 membranes�The performance of the IMB was further improved by in situ adsorption and the highest product titer of 709 mg/L was achieved in the IMB with an addition of 10 g/L sp-207 resin at 0 h of fermentation,which was 660% of that in the SCB�The volumetric productivity of 30 mg/( L·h) was also higher than that of the 6 mg/( L·h) in the SCB�The hollow fiber membranes in the IMB served as a carrier to immobilize cells and a second phase to sequester insoluble reactant,thus reducing inhibition of substrate as well as products and facilitating a more effective biotransformation by combining with the advantages of the immobilized-cell bioreactor and the two-phase bioreactor.