利用二次通用旋转组合设计方法，研究了菜蛾盘绒茧蜂Cotesiavestalis滞育茧在不同正己烷浓度、不同温度下暴露不同时间后对该蜂滞育解除、以及滞育解除后成虫羽化率和成蜂繁殖力的影响，分别建立了茧．成虫羽化历期、羽化率、单雌产卵量对正己烷浓度、暴露时间、温度3因子的回归模型。结果表明：正己烷可以有效促进该蜂解除滞育，显著缩短羽化历期，提高羽化整齐度；但正己烷浓度、暴露时间和温度水平的过高或过低在一定程度上会降低滞育解除后成蜂的繁殖力，过高的温度处理还会显著降低成虫的羽化率。基于回归模型的优化组合分析结果，提出将滞育茧置于27-29°C下、1．5mL·L。的正己烷蒸气中暴露10～15min，对该蜂解除滞育后的生物学特性的不良影响将会降至最低。%Effects of different n-hexane concentrations, temperatures and exposure time on larval diapause termination of Cotesia veLrtalis, and its post-diapause emergence and fecundity were investigated by quadratic general rotary unitized design. The regression model of each parameter, that is duration from cocoon to adult emergence, emergence rate and eggs laid per female, and the three factors were established, respectively. Results indicated that n-hexane could accelerate the diapause termination of the parasitoid, shorten the cocoon-adult duration, and synchronize the adult emergence. However, too high or low levels of n-hexane concentration, exposure time and temperature would reduce adult fecundity post diapause termination in some degree and too high level of temperature would significantly reduce emergence rate of the wasp. Based on the comprehensive analysis of the models, exposing the diapausing individuals under the combination of 27-29 °C and 1.5 mL-L-1 n-hexane for 10-15 rain would effectively terminate the diapause with the least adverse effects on the biological characteristics of the oarasitoid.
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