为防治近几年越冬茬黄瓜上的新病害——细菌性茎软腐病,从海边盐生植物根际土壤中富集培养、分离和鉴定了产1-氨基环丙烷-1-羧酸(ACC)脱氨酶的植物根际促生菌(PGPR),共鉴定出了嗜麦芽寡养单胞菌Stenotrophomonas maltophilia、土生拉乌尔菌Raoultella terrigena、土壤杆菌Agrobacterium tumefaciens、产酸克雷伯菌Klebsiella oxytoca、成团泛菌Pantoea agglomerans、产气肠杆菌Enterobacter aerogenes和克吕沃尔氏菌Kluyvera cryocrescens共7个种的PGPR.测定了ACC脱氨酶活性,其中嗜麦芽寡养单胞菌菌株CRG-2,产生ACC脱氨酶活性最高,为324.3 μmol/(mg.h).将菌株CRG-2和枯草芽胞杆菌复配制成丸化种衣剂后,测定了对黄瓜细菌性茎软腐病的田间防治效果.结果表明,嗜麦芽寡养单胞菌和枯草芽胞杆菌复配后形成的种衣剂对黄瓜细菌性茎软腐病的田间防治效果达到73.13％,高于中生菌素防治效果15.79％.由此可见该种衣剂有望在生产上用于防治黄瓜细菌性茎软腐病.%The plant growth promoting rhizo bacteria (PGPR) producing 1-amino cyclopro-pane-l-carboxylic acid (ACC) deaminase were isolated and identified from the rhizosphere soil of seashore halophytesin order to prevent and control cucumber bacterial stem soft rot,a new disease in winter greenhouse cucumber in recent years.7 species of PGPR including Stenotrophomonas maltophilia,Raoultella terrigena,Agrobacterium tumefaciens,Klebsiella oxytoca,Pantoea agglomerans,Enterobacter aerogenes and Kluyvera cryocrescens were identified.The activity of ACC deaminase in Stenotrophomonas maltophilia CRG-2 was the highest,324.3 μmol/(mg·h) in 22 strains.At the same time,the biocontrol effect of biological seed-coating by mixing isolate strain CRG-2 and Bacillus subtilis with additives to cucumber bacterial stem soft rot were tested in the field.The control effect of the seed coating agent formed by the mixture of strain CRG-2 and Bacillus subtilis to cucumber bacterial stem soft rot reached 73.13％ in the field,higher than zhongshengrnycin with control effect of 15.79％.The encouraging effect of seeding coat on biological control suggested that it had the potential to be applied widely in the cucumber bacterial stem soft rot control in the future.
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