Purpose To investigate the anti-proliferative effects of curcumin on human hepatocellular carcinoma HepG2 and Bel-7404 cells. Methods In this experiment were set up the groups of different concentrations curcumin (2.5,10.0,25.0,50.0 and 100.0 μg/mL),control group(0.2% DMSO +RP-MI 1640 medium + cells) and 2 μg/mL cisplatin group. The hepatocellular carcinoma HepG2 and Bel-7404 cells were detected by MTT method. Results The anti-proliferation of curcumin had a dose-effect relationship and was in time-dependent manner. Under the condition of the same reaction time,the anti-proliferative effects of curcumin and cisplatin were different significantly (P 0.05) ,and its anti-proliferative effects in 25.0,50.0,and 100.0 μg/mL were significantly different(P < 0.05). Conclusion Curcumin could significantly inhibit the proliferation of human hepatocellular carcinoma HepC2 and Bel-7404 cells in vitro.%目的 研究姜黄素对人肝癌HepG2和Bel-7404细胞增殖的影响.方法 不同浓度的姜黄素(2.5,10.0,25.0,50.0,100.0μg/mL),0.2％二甲基亚砜的RPMI 1640培养基加细胞为空白对照,2μg/mL顺铂为阳性对照.MTT法检测肝癌HepG2和Bel-7404细胞的增殖.结果 姜黄素对肝癌细胞HepG2和Bel-7404的增殖抑制有量效关系,并呈时间依赖性.在相同作用时间下,姜黄素组、顺铂组对肝癌细胞HepG2和Bel-7404增殖有明显的抑制作用,与空白对照组比较均有显著性差异(P＜0.05).与空白对照组比较,2.5,10.0 μg/mL姜黄素对肝癌细胞HepG2和Bel-7404的增殖没有明显的抑制作用(P＞0.05),而25.0,50.0,100.0μg/mL姜黄素以及顺铂组对肝癌细胞HepG2和Bel-7404的增殖均有明显的抑制作用(P＜0.05).结论 姜黄素可以明显的抑制肝癌细胞HepG2和Bel-7404的生长,且存在剂量-时间的关系.
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