富硒化对博落回有效成分抑菌效果的影响研究

摘要

Objective To study on antibacterial effect of active ingredient of se-enriched Macleya cordata.Methods Penicillin sodium and saline as control, detected the average inhibition zone diameter of se-enriched Macleya cordata group ( containing 0.96 mg/mL sanguinarine) on 9 common bacteria with disc agar diffusion method;20% methanol and saline as control, se-enriched Macleya cordata group which the average inhibition zone diameter was minimum, without se-enriched Macleya cordata group and penicillin sodium were diluted with 20% methanol, made concentration of 0.48, 0.24,0.12,0.06, 0.03 mg/mL.minimal inhibitory concentration ( MIC) of each treatment group was determined by agar dilution method, evaluated antibacterial effect of se-enriched Macleaya cordata group.Results se-enriched Macleya cordata group could improve antibacterial effect significantly, the average inhibition zone of 100 μg/mL sodium selenite treatment group was minimum(Φ15.33 mm);MIC of se-enriched Macleya cordata group on E.coli O1 , E.coli P22 , E.Dublin reduced from 0.96 to 0.24 mg/mL;MIC of S.typhimurium and S.suipare were 0.12 mg/mL;MIC of S.aureus and S.suis were 0.06 mg/mL; MIC of E.Dublin and L.murrayi were less than 0.03 mg/mL.Conclusion Se-enriched Macleya cordata improves the antibacterial effect signififcantly, and it lays the theoretical foundation for development and utilization of se-enriched Macleya cordata for future.%目的：研究富硒化对博落回姚效成分抑菌效果的影响。方法以青霉素钠、生理盐水为对照，采用琼脂扩散纸片法检测各富硒化博落回组处理9种常见细菌的平均抑菌圈直径；以20％甲醇、生理盐水作为对照，用20％的甲醇将平均抑菌圈最小的富硒化博落回组，无硒处理博落回组和青霉素钠组分别稀释，使浓度均为0．48、0．24、0．12、0．06、0．03 mg／mL，采用琼脂平板稀释法测定其MIC，评价对其9种常见细菌的抑制作用。结果富硒化处理能大幅提高博落回生物碱的抑菌效果，100μg／mL亚硒酸钠处理组的平均抑菌圈最小（15．33 mm）；富硒化博落回生物碱对大肠杆菌O1、大肠杆菌P22、都伯林大肠杆菌的最低抑菌浓度为从原来0．96 mg／mL降低至0．24 mg／mL；对鼠伤寒杆菌、猪霍乱杆菌的最低抑菌浓度为0．12 mg／mL；对金黄色葡萄球菌、猪链球菌的最低抑菌浓度为0．06 mg／mL；对枯草芽孢杆菌和李氏杆菌的最低抑菌浓度小于0．03 mg／mL。结论富硒化显著改善博落回的抑菌效果，为今后富硒博落回的开发利用，奠定了理论基础。