目的 观察中药姜黄素对乳腺癌SKBR3细胞株增殖、凋亡、Caspase-3活性及端粒酶的影响,探讨姜黄素的作用机制.方法采用MTT法绘制细胞生长曲线;PI荧光和Annexin V荧光双染检测凋亡细胞;采用Westernblot法检测Caspase-3的表达;采用TRAP-PCR银染法检测端粒酶活性.结果姜黄素对SKBR3细胞增殖具有明显的抑制作用,呈时间和浓度依赖性,对SKBR3细胞端粒酶活性具有一定的降低作用,可提高Caspase-3表达水平,诱导细胞凋亡.结论姜黄素能够有效抑制SKBR3细胞增殖,增加Caspase-3蛋白的合成,抑制端粒酶的活性而介导SKBR3细胞凋亡.%Objective To observe the effect of curcumin on proliferation, apoptosis, Caspase-3 activity and telomerase activity of chronic granulocytic breast cancer SKBR3 cell line, and to investigate the mechanism of curcumin. Methods The cell growth curve was drawn by MTT;the apoptotic cells were detected by Annexin, V and PI double staining; the expression of Caspase-3 was detected by Westernblot; the activity of telomerase was detected by TRAP-PCR silver staining. Results Curcumin could significantly inhibit the proliferation of SKBR3 cells in a time and concentration dependent, has a certain effect on reducing the telomerase activity of SKBR3 cells can enhance the expression of Caspase-3 induced apoptosis. Conclusion Curcumin can effectively inhibit the proliferation of SKBR3 cells, increase the synthesis of Caspase-3 protein, inhibit the activity of telomerase and mediate the apoptosis of SKBR3 cells.
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