基于小动物超声的小鼠Lewis肺癌胸腔积液模型的建立

摘要

目的 建立C57BL/6小鼠Lewis肺癌胸腔积液的超声检测模型.方法 将Lewis肺癌的肿瘤组织制作成单细胞瘤液,接种到C57BL/6小鼠胸腔内,采用小动物超声的检测方法,观察小鼠瘤性胸腔积液的形成,并抽取小鼠胸腔积液,计算瘤液体积及胸腔积液中肿瘤细胞数量.结果 将Lewis肺癌细胞接种到小鼠胸腔后,小鼠均可产生肺癌晚期胸腔积液,小动物超声下观察到小鼠胸腔内有明显液性暗区和均匀回声,随时间延长暗区及均匀回声增多,解剖动物胸腔内有大量血样胸腔积液.采用化疗药物环磷酰胺和顺铂可减少肺癌胸腔积液体积.结论 基于小动物超声的小鼠Lewis肺癌胸腔积液模型可用于评价药物对于肺癌胸腔积液的疗效.%Objective To establish a malignant pleural effusion model of Lewis lung cancer in C57BL/6 mice based on Micro Echo technology. Methods Single tumor cell suspension of Lewis lung cancer was injected into thoracic cavity.The pleural effusion was detected by Micro Echo technology.The volumes of effusion were quantified and the tumor cells were counted.Results After implanted of tumor cell, malignant pleural effusion can be detected by Micro Echo technology and observed after autopsy.Chemotherapy drugs such as Cyclophosphamide and Cisplatin can decrease the effusion volumes.Conclusion Pleural effusion model of Lewis lung cancer based on Micro Echo technology can be used to evaluate the efficacy of antitumor drugs.