“和血生络法”对心梗大鼠血清中VEGF的影响

摘要

Objective: To observe the Effect of Hexue ShengluoFa on VEGF. To investigate the mechanism of Hexue Shengluofa on advancing cardiac muscle's Angiogenesis of myocardium of imfarcted rats. It is the science base of prevention and cure of coronary heart disease. Methods: Myocardium of imfarcted rats model were made by Wistar rats. They were divided randomly into four groups which were model group, ID group, Hexue Shengluo high and low dose groups. Sham operation group was made at the same time. Every group had 12 rats. Subsequently drug treatment groups were administrated with ID water solution in accordance with 2. 7mg · kg-1 · d-1 and with Hexue Shengluo water solution in accordance with 9. 9g · kg-1 · d-1,19.8g · kg-1 · d -1. The model group and sham group were treated with distilled water with the equal volume. Rats were treated once every day by intragastric administration. After 4 weeks continuously, executed the rats. The content of serum VEGF was evaluated. Results; The VEGF content of All treatment groups were higher than that of model group (P < 0.05 ) . High-dose group was better than low - dose group and ID group ( P < 0. 05 ). Conclusion: The mechanism to promote angiogenesis of Hexue Shengluofa may be generated by promoting VEGF realize.%目的:通过观察“和血生络方”对VEGF的影响,探讨“和血生络法”促进心肌梗死大鼠心肌血管新生的作用机制,从而为“和血生络法”防治冠心痛提供科学依据.方法:选雄性Wistar大鼠制作心肌梗死大鼠模型,随机分为消心痛组、和血生络高、低剂量组及模型组,同时建立假手术组,每组12只.药物治疗组分别给予和血生络水溶液(9.9g·kg-1·d-1,19.8g·kg-1·d-1)及消心痛水溶液[2.7mg·kg-1·d-1]每天1次灌胃,其他组给予等量蒸馏水,连续用药4周后处死大鼠,检测血清中VEGF的含量.结果:和血生络高、低剂量组及消心痛组血清中VEGF含量明显高于模型组(P＜0.05),和血生络高剂量组优于其它处理组(P＜0.05).结论:“和血生络法”促血管新生的机制可能是通过促进VEGF生成实现的.