熟地寄生壮骨方对大鼠创伤性骨关节炎模型抗炎及保护软骨作用的实验研究

摘要

目的:观察熟地寄生壮骨方对大鼠创伤性骨关节炎模型的影响,探讨其抗炎及保护软骨作用.方法:采用创伤性膝骨关节炎大鼠模型,切取病变膝关节制备膝关节匀浆液,测定给药前后一氧化氮(N0)、肿瘤坏死因子-a(TNF-a)的含量;病变膝关节组织病理切片,按Mankin骨关节炎组织分类法进行评分.结果:与空白组比较,模型组大鼠膝关节NO、TNF-α含量显著增大,大鼠膝骨关节Mankin评分值显著增大;与模型组比较,熟地寄生壮骨方各组NO、TNF-α含量均明显减小,大鼠膝骨关节Mankin评分值均明显减小.结论:熟地寄生壮骨方能降低创伤性骨关节炎模型大鼠膝关节NO、TNF-α含量及改善其软骨形态,达到控制炎症及保护软骨的目的.%Objective:To observe the anti-inflammatory effect and the morphology change of articular cartilage under intervention of Shudi Jisheng Zhuanggu decoction (SJZ) in knee post-traumatic osteoarthritis model rats.Methods:Rats were removed the medial collateral ligament and the medial meniscus to build models of knee post-traumatic osteoarthritis (Hulth's modeling method)before administration of drugs.Prepared 20％ homogenization buffer of the knees to measure the contents of nitric oxide (NO) and TNF-α.Histologic sections of cartilage were received HE staining and Gemini staining were evaluated according to Mankin cartilage histological score.Results:Mankin score and the contents of NO and TNF-α were significantly increased in model group compared to control group.Mankin score and contents of NO and TNF-α of SJZ groups were significantly decreased compared to model group.Conclusion:SJZ can control inflammation and ameliorate morphology of articular cartilage in rats with knee Post-traumatic osteoarthritis,so as to protect the cartilage and delay cartilage degeneration.