响应面法优化超声波辅助提取米尔贝链霉菌中米尔贝霉素A3和A4的工艺研究

摘要

This paper reported the use of response surface methodology to improve the yields of milbemycin A3 and A4 from Streptomyces milbemycinicus obtained by ultrasonic-assisted extraction.The yields of milbemycins extracted under the optimized conditions were characterized by reversed phase high performance liquid chromatography.A three-factors-three-levels Box-Behnken design was conducted to optimize extraction time (X1),ultrasonic power (X2) and duty cycle (X3) for the optimal yields of milbemycin A3 (Y1) and milbemycin A4 (Y2).The studies indicated that the optimal ultrasonic-assisted extraction conditions were ethyl acetate,the biomass to solvent ratio 0.8,extraction time 30min,extraction power 110W,the duty cycle 40％,the extraction temperature 10℃,giving a maximum extraction yield of 312.5 and 174mg/g of the milbemycin A3 and A4,respectively,which were about 2.3 and 2.5 fold increase in the yield as compared to conventional extraction methods.%本文报道了超声波辅助提取米尔贝链霉菌中米尔贝霉素A3和A4的产量的方法,采用反向高效液相表征米尔贝霉素A3和A4的产量,并通过响应面法来优化提取工艺.对提取时间(X1)、超声功率(X2)和占空比(X3)进行了三因素三水平Box-Behnken设计,以获得米尔贝霉素A3(Y1)和A4(y2)的最佳产量.研究表明,最佳超声波辅助提取条件为:溶剂为乙酸乙酯,料液比0.8,提取时间30min,超声功率110W,占空比40％和提取温度10℃.在此提取条件下米尔贝霉素A3和A4的最大产量分别为312.5和174mg/g,分别是传统提取方法产量的2.3和2.5倍.