内皮素-1(ET-1)对羊驼皮肤黑素细胞增殖和黑素生成的影响

摘要

旨在研究内皮素-1 (Endothelin-1,ET-1)对羊驼皮肤黑素细胞(Melanocyte,MC)增殖和黑素合成的影响.本研究中,体外培养正常羊驼皮肤黑素细胞,观察不同浓度ET-1(0、0.1、1、10、100 nmol·L-1)对羊驼皮肤黑素细胞增殖、黑素含量、内皮素受体B(Endothelin recepter B,EDNRB)基因、酪氨酸酶(Tyrosinase,TYR)基因、酪氨酸相关蛋白-1(Tyrosinase related protein 1,TRP-1)基因和表皮黑皮素1受体(Melanocortin 1 receptor,MC1R)基因表达量的影响.结果表明,ET-1处理羊驼皮肤黑素细胞3d后,羊驼皮肤黑素细胞增多,黑素含量、EDNRB、TRP-1和TYR基因表达量都明显增加(P＜0.05),以10 nmol·L-1组最为显著.ET-1能诱导羊驼皮肤黑素细胞增殖、树突增长,诱导EDNRB、TYR和TRP-1基因表达量增高,使黑素合成增加；同时诱导MC1R基因表达量增高,从而通过α-MSH信号通路对羊驼黑色素的生成产生影响.%To study the effects of Endothelin-l(ET-l) on the proliferation and melanin synthesis of cultured alpaca skin melanocytes, the cultured alpaca skin melanocytes were treated with various concentrations of ET-KO, 0.1, 1, 10, 100 nmol · L-1) in vitro. The effects of ET-1 on mel-anocyte proliferation, melanin contents, EDNRB, TYR, TRP-1 and MC1R gene expeession in cultured melanocytes were observed. Three days after treatment with ET-1, the number of melanocytes increased and melanin contents, EDNRB, TYR and TRP-1 gene expression levels also remarkably increased in treated cells compared to those in untreated cells(P<0. 05), especially when the concentration was 10 nmol · L-11. The result indicate that ET-1 can induce the dendrites elongated, proliferation and the expression of EDNRB, TYR and TRP-1, and induce the melanin synthesis; Also, ET-1 induce the expression of MC1R gene, and affect the melanin synthesis of alpaca by the pathway of a-MSH.