基于通路分析方法诠释肉鸡体重性状全基因组关联研究

摘要

本研究旨在通过对体重和日增重进行基于通路的关联分析,以期获得影响体重和日增重的信号通路.对北京油鸡与科宝肉鸡F2资源群体14、28、56、93日龄体重及0～14、14～28、28～56、56～93日龄日增重分别进行全基因组关联分析,利用关联分析获得的显著性SNP位点进行基于通路的关联分析(SNP ratio test).结果表明,14日龄体重与类固醇激素的生物合成相关联(P＜0.05),56日龄体重与烟酸/烟酰胺的代谢相关联(P＜0.05),93日龄体重与糖酵解/糖异生相关(P＜0.05),且各日龄体重性状均与氨基酸的代谢过程相关联(P＜0.05).在增重最快的42～56日龄,与日增重相关的SNP富集到的通路最多,这些通路主要集中在TCA循环、类固醇合成、类固醇激素的生物合成、氧化磷酸化、磷酸肌醇代谢、甘油磷脂代谢等过程.在肉鸡体重迅速增长阶段类固醇激素的生物合成起到了重要作用,且氨基酸代谢过程与日增重显著相关.%To identify the pathways associated with body weight and average daily gain,we conducted the genome-wide association study(GWAS) and SNP ratio test(SRT) of F2 populations derived from a local Chinese breed (Beijing-You chickens) and a commercial fast-growing broiler line (Cobb-Vantress).In this study,the body weight of F2 chickens were measured at 14-day-old,28-day-old,56-day-old and 93-day-old,and the average daily gains of each period were calculated,respectively.The GWAS of body weight and average daily gain were carried out.The SRT was performed using the significant SNPs obtained from the GWAS.The results showed that biosynthesis of steroid hormones,metabolism of nicotinic acid and glycolysis/gluconeogenesis were genome-wide significantly associated with 14-day-old,56-day-old and 93-day-old body weight (P＜0.05),respectively.Specially,amino acid metabolic processes were also associated with the body weight(P＜0.05) in each period.The enriched pathways by SNPs associated with daily gain were the most abundant in 42-56 days,these pathways were enriched in TCA cycle,steroid biosynthesis,steroid hormone biosynthesis,oxidative phosphorylation,inositol phosphate metabolism and glycerophospholipid metabolism.In brief,biosynthesis of steroid hormones plays an important role in the rapid growth of body weight of broiler,and a large number of amino acid metabolic processes are also associated with average daily gain.