苷元型大豆异黄酮对母羊氧化应激、乳中免疫球蛋白和羔羊生长的影响

摘要

This study aimed to investigate the effects of diet supplemented with different amounts of isoflavone aglycones on the oxidative stress parameters,immunoglobulin in milk of ewes and growth of lambs during late pregnancy to lactation.A that of 33 female Chongming white goats on day 100 of gestation (with similar age,weight and parity) were randomly divided into 3 groups.The control group (C0 group,11 ewes) was fed with basal diet while the experiment groups were supplemented with 20 mg/kg (T1 group,9 ewes) and 30 mg/kg (T2 group,13 ewes) isoflavone aglycones in the basal diet respectively.The results showed that:The litter size,the litter birth weight,the litter weaning weight and the litter daily gain in T1 group and T2 group were significantly higher than those in C0 group (P＜0.05);On the 1st day of lactation,the level of serum H2O2 of ewes in T2 group was significantly lower than that in C0 group and T1 group (P＜0.05).Compared to T1 group and T2 group,T-SOD activity of serum in C0 group was the highest (P＜0.05),and T-AOC was the lowest(P＜0.05).There was no significant difference in MDA among the three groups (P＞0.05).On the 21 st day of lactation,the level of serum H2O2 of ewes in T2 group was significantly lower than that in C0 group and T1 group (P＜0.05).Compared to C0 group and T2 group,T-SOD activity of serum of T1 group was the highest (P＜0.05),and there was no significant difference in T-AOC and MDA among the three groups (P＞0.05);The amount of immunoglobulin A in colostrum in T1 group and T2 group was significantly higher than that in C0 group (P＜0.05).The results suggested that diet supplemented with isoflavone aglycones to ewes during late pregnancy to lactation could effectively reduce oxidative damage,increase the amount of immunoglobulin in colostrum and promote the growth of lambs.Dietary supplementation with 30 mg/kg isoflavone aglycones was more significant.%本试验旨在研究日粮中添加不同水平的苷元型大豆异黄酮对妊娠后期及泌乳期母羊氧化应激、乳中免疫球蛋白和羔羊生长的影响.试验选用33只年龄、体重、胎次相近的妊娠100 d的崇明白山羊,随机分为3组,对照组饲喂基础日粮(C0组,11只),试验组分别在基础日粮中添加20 mg/kg(T1组,9只)和30 mg/kg (T2组,13只)苷元型大豆异黄酮.结果表明:T1和T2组母羊的窝产羔数、初生窝重、断奶窝重和窝日增重显著高于C0组(P＜0.05);分娩当日,T2组母羊血清中过氧化氢(H2O2)含量显著低于C0和T1组(P＜0.05);与T1和T2组相比,C0组血清中总超氧化物歧化酶(T-SOD)活性最高(P＜0.05),总抗氧化能力(T-AOC)最低(P＜0.05),3组间丙二醛(MDA)无显著差异(P＞0.05);在泌乳21d,T2组母羊血清中H2O2含量显著低于C0和T1组(P＜0.05);与C0和T2组相比,T1组血清中T-SOD活性最高(P＜0.05),3组的T-AOC和MDA无显著差异(P＞0.05);T1和T2组初乳中免疫球蛋白A(IgA)含量显著高于C0组(P＜0.05).结果提示,在妊娠后期及泌乳期母羊饲粮中添加苷元型大豆异黄酮可有效减缓母羊的氧化损伤,提高初乳中免疫球蛋白含量,促进羔羊生长发育,且添加量以30 mg/kg效果更显著.