高钙饲粮对青年蛋鸡血清一氧化氮浓度和抗氧化功能的影响

摘要

本试验旨在研究高钙饲粮对青年蛋鸡血清一氧化氮(NO)浓度和抗氧化功能的影响.将35日龄100羽伊沙蛋鸡随机均分为对照组(含钙1.00％)和高钙组(含钙3.78％),每组5个重复,每个重复10羽.每天观察鸡的精神状态、食欲和粪便情况.在饲喂高钙饲粮第0、8、16、24和32天时,分别测定2组鸡的血清中NO浓度和丙二醛含量及抗氧化酶系统的变化.结果表明:1)高钙饲粮能引起青年蛋鸡长期腹泻,在饲喂高钙饲粮的第8天就发生高血钙症.2)与对照组相比,高钙组在饲喂高钙饲粮第8天和第16天血清NO浓度无显著变化(P＞0.05),第24天和第32天显著升高(P＜0.05);第8天和第24天血清超氧化物歧化酶活性、总抗氧化能力无显著变化(P＞0.05),第16天显著升高(P＜0.05),第32天显著降低(P＜0.05);第8、16、24天血清丙二醛含量无显著变化(P＞0.05),第32天显著升高(P＜0.05);第24天和第32天黄嘌呤氧化酶活性有升高趋势(P＞0.05).结果提示,饲喂高钙饲粮可导致青年蛋鸡发生长期腹泻,引起高血钙症,导致其机体内自由基的产生与清除的动态平衡遭到破坏,使自由基的产生远远超出清除能力.%This experiment was conducted to study the effects of high calcium diet on nitric oxide (NO) concentration and antioxidant function of pullets. One hundred healthy pullets aged 35 days were randomly divided into 2 groups with 5 replicates in each group and 10 chickens per replicate. The pullets in different groups were fed normal calcium diet (control group, 1. 00% calcium) and high calcium diet (HC group, 3. 78% calcium) , respectively. Healthy situations of the pullets were inspected daily. NO concentration, malondialdehyde (MDA) content and enzymatic antioxidant activity in serum were detected on days 0, 8, 16, 24 and 32 after feeding high calcium diet. The results showed as follows: 1) high calcium diet could cause diarrhea. Pullets would be hypercalcemia on day 8 after feeding high calcium diet. 2) There was no significant difference in serum NO concentration of pullets between HC group and the control group on days 8 and 16 after feeding high calcium diet (P > 0. 05) , but that in HC group was significantly increased on days 24 and 32 after feeding high calcium diet (P < 0. 05). There were no significant differences in serum superoxide dismutase ( SOD) activity and total anti-oxidation capability (T-AOC) of pullets between HC group and the control group on days 8 and 24 after feeding high calcium diet (P >0. 05) , but those in HC group were increased on day 16 after feeding high calcium diet (P <0. 05) , meanwhile, decreased on day 32 after feeding high calcium diet compared with the control group (P < 0. 05). There was no significant difference in serum MDA content of pullets between HC group and the control group on days 8, 16 and 24 after feeding high calcium diet (P > 0.05) , but that in HC group was significantly increased on day 32 after feeding high calcium diet ( P < 0. 05). There was a increasing trend in serum xanthine oxidase (XOD) activity in HC group on days 24 and 32 after feeding high calcium diet compared with the control group (P > 0. 05). The results indicate that high calcium diet can cause long-term diarrhea, increase the lipid peroxidation, and decrease the enzymatic antioxidant activity.