This experiment was aimed to study the effects of corn stalks treated with seven different microbes and their combinations. Corn stalks were fermented with 1 strain( Phanerochaete chrysosporium and Penicilli-um sp. ),2 strains( Phanerochaete chrysosporium+ Aspergillus niger and Phanerochaete chrysosporium+ Tri-choderma viride),3 strains( Phanerochaete chrysosporium+Aspergillus niger+Penicillium sp. and Phanero-chaete chrysosporium+Aspergillus niger+Trichoderma viride)or 4 strains( Phanerochaete chrysosporium+As-pergillus niger+Trichoderma viride+Penicillium sp. )of microorganisms( seven combinations in total),and six Dorper×Thin-tailed crossbred castrated sheep F1 were used. In situ method was applied to measure the dynamic rumen degradation rate of dry matter,organic matter,neutral detergent fiber,acid detergent fiber,acid deter-gent lignin,cellulose and hemicellulose at 0,6,12,24,48 and 72 h. The results showed that microbes im-proved the effective degradation rate of neutral detergent fiber,acid detergent fiber,acid detergent lignin,cel-lulose,hemicellulose,and crude protein of corn stalks( P<0.05). The effects of different microbes and their combinations were inconsistent. The combination of Phaerochaete chrysosporium+Trichoderma viride is superi-or to other strains or combinations. The utilization efficiency of corn stalks can be improved by using certain combination of microbes.%本试验旨在研究7种不同微生物菌株及其组合处理玉米秸秆的效果。选取单菌株（黄孢原毛平革菌、青霉菌）、2菌株（黄孢原毛平革菌＋黑曲霉菌、黄孢原毛平革菌＋绿色木霉菌）、3菌株（黄孢原毛平革菌＋黑曲霉菌＋青霉菌、黄孢原毛平革菌＋黑曲霉菌＋绿色木霉菌）和4菌株（黄孢原毛平革菌＋黑曲霉菌＋绿色木霉菌＋青霉菌）共计7个组合处理玉米秸秆，并设置对照组。利用6只装有瘤胃瘘管的杜×寒F1代杂交羯羊，采用半体内法分别对玉米秸秆的干物质、有机物、中性洗涤纤维、酸性洗涤纤维、酸性洗涤木质素、纤维素和半纤维素在0、6、12、24、48、72 h的动态降解率进行测定与分析。结果表明：玉米秸秆经微生物菌株处理，其中性洗涤纤维、酸性洗涤纤维、酸性洗涤木质素、纤维素、半纤维素、粗蛋白质的有效降解率显著高于对照组（P＜0．05），不同菌株及其组合的处理效果不一致。黄孢原毛平革菌＋绿色木霉菌组合的效果优于其他菌株或组合。用合理的微生物组合处理玉米秸秆可以有效提高其利用率。
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