猪源唾液乳杆菌对生长猪生长性能、粪中微生物数量及血清指标的影响

摘要

本试验旨在探讨猪源唾液乳杆菌( L. salivarius)对生长猪生长性能、粪中微生物数量及血清指标的影响。选用平均体重为(35.22±0.86) kg的长×大二元杂交生长猪81头，随机分成3组，每组3个重复，每个重复9头。对照组饲喂基础饲粮，试验组分别饲喂在基础饲粮中添加0.2%、0.4%(每克饲粮活菌数分别为7.0×106和1.4×107 CFU)的猪源L. salivarius冻干制剂。试验期为30 d。结果表明：1)与对照组相比，试验组生长猪的平均日采食量( ADFI)和料重比(F/G)无显著变化(P>0.05)，平均日增重(ADG)有升高趋势(P>0.05)；2)饲粮中添加0.2%、0.4%的猪源L. salivarius能够显著增加生长猪粪中乳酸菌数量( P<0.05)，显著降低大肠杆菌数量(P<0.05)，并显著提高乳酸菌与大肠杆菌的比值(P<0.05)；3)与对照组相比，试验组生长猪血清总胆固醇( TCHO)含量及谷丙转氨酶( ALT)、谷草转氨酶( AST)活性显著降低(P<0.05)；4)试验组生长猪血清结合珠蛋白(Hp)含量均较对照组显著降低(P<0.05)。结果提示，饲粮中添加猪源L. salivarius能够改善生长猪肠道菌群环境和血清指标，提高机体免疫力，进而促进其健康生长。%This experiment was conducted to study the effects of L. salivarius from pigs on growth perform-ance, the number of faecal microflora and serum indices of growing pigs. Eighty-one crossbred ( Landrace × Large White) growing pigs with an average body weight of (35. 22 ± 0. 86) kg were divided into 3 groups with 3 replicates per group and 9 pigs per replicate. Pigs in control group were fed a basal diet and those in ex-perimental groups were fed experimental diets supplemented with 0 . 2% and 0 . 4% ( viable bacteria counts were 7. 0 × 106 and 1. 4 × 107 CFU per gram of diets, respectively) lyophilized preparation of L. salivarius from pigs, respectively. The experiment lasted for 30 days. The results showed as follows:1) compared with the control group, the average daily feed intake ( ADFI) and feed/gain ( F/G) of growing pigs in the experi-mental groups had no significant changes ( P >0 . 05 ) , and the average daily gain ( ADG ) had increasing trends ( P>0 . 05 );2 ) the supplementation of L. salivarius at 0 . 2% and 0 . 4% could significantly increase the number of Lactobacilli (P<0. 05), significantly reduce the number of E. coli (P<0. 05), and signifi-cantly increase the ratio of Lactobacilli to E. coli in the faeces (P <0. 05); 3) compared with the control group, the activities of alanine transferase ( ALT ) and aspartate transferase ( AST ) and the content of total cholesterol (TCHO) in serum in the experimental groups were significantly decreased (P<0. 05); 4) com-pared with the control group, the content of haptoglobin ( Hp) in serum in the experimental groups was signifi-cantly decreased ( P<0 . 05 ) . The results indicate that the supplementation of L. salivarius from pigs can im-prove the environment of intestinal microflora and the indices of serum, increase the immunity of body, and then advance the growth of growing pigs.