手工计数质控不同实验室计算机辅助精液分析检测精子浓度的初步研究

摘要

目的 研究通过手工计数精子浓度质控不同实验室计算机辅助精液分析(CASA)检测精子浓度,以保证不同实验室之间检测的精子浓度标准的统一性.方法 2016年1月1日至2016年3月31日,通过手工计数和CASA两种方法计数3家医院共600份精液的精子浓度,通过分析比较手工计数与CASA检测结果的相关性.根据手工计数的结果,对于CASA的相关参数进行相应校正.2016年4月1日至2016年6月30日,再次通过手工计数和CASA两种方法计数3家医院共600份精液的精子浓度,分析比较手工计数与CASA检测的结果.结果 (1)2016年1月1日至2016年3月31日,检测的600份精液标本,其中同济大学附属第一妇婴保健院400份,手工计数和CASA两种方法检测精子浓度,差异没有统计学意义(P>0.05);上海市浦东新区妇幼保健院100份,手工计数的结果高于CASA检测的结果,差异具有统计学意义(P<0.05);上海市闵行区妇幼保健院100份,手工计数的结果低于CASA检测的结果,差异具有统计学意义(P<0.05).(2)根据以上检测结果,对于CASA的相关参数进行相应校正.2016年4月1日至2016年6月30日,检测的600份精液标本,其中同济大学附属第一妇婴保健院400份,手工计数和CASA两种方法检测精子浓度,差异没有统计学意义(P>0.05);上海市浦东新区妇幼保健院100份,差异没有统计学意义(P>0.05);上海市闵行区妇幼保健院100份,差异没有统计学意义(P>0.05).结论 通过对于不同实验室手工计数精子浓度的标准统一化,来定期校正CASA的检测结果,以达到保证不同实验室之间CASA检测精子浓度的准确性和一致性.%Objective To investigate quality control (QC) of Computer-aided Sperm Quality Analysis(CASA) in different laboratories for counting sperm concentration by manual counting, and ensure a standard of sperm concentration counting between different laboratories. Methods Sperm concentrations of 600 semen samples in three different hospitals were determined by manual counting and CASA from January 1, 2016 to March 31, 2016, the CASA parameters wereadjusted based on the results of manual counting. Subsequently, sperm concentrations of other 600 semen samples in three different hospitals were determined by manual counting and CASA from April 1, 2016 to June 30, 2016. The results were comparatively analyzed. Results (1) From January 1, 2016 to March 31, 2016, there was no difference in the results of sperm concentration of 400 semen samples from Shanghai First Maternity and Infant Hospital between manual counting and CASA (P>0.05); the level of sperm concentration in 100 semen samples from Children and Women's Health Care Hospital in Pudong New Area using manual counting was higher than that using CASA (P<0.05); the level of sperm concentration in 100 semen samples from Children and Women's Health Care Hospital in Minhang Area using manual counting was lower than that using CASA (P<0.05). (2) CASA parameters in different laboratories were adjusted based on the above results. from April 1, 2016 to June 30, 2016, there was no difference in the results of sperm concentration of 400 samples from Shanghai First Maternity and Infant Hospital between manual counting and CASA (P>0.05). There was no difference in the results of sperm concentration of 100 samples from Children and Women's Health Care Hospital in Pudong New Area between manual counting and CASA (P>0.05). There was no difference in the results of sperm concentration of 100 samples from Children and Women's Health Care Hospital in Minhang Area between manual counting and CASA (P>0.05). Conclusion The accuracy and consistency of sperm concentration counting between different laboratories can be significantly improved by adjusting the CASA parameters according to the results of sperm concentration by standard manual counting regularly.