Methamphetamine induces cireTLk1 expression in cultured neurons and addiction mouse

摘要

Circular RNA is a type of non-coding RNA which forms a loop structure.CircRNA was reported to exhibit cell-and tissue-specific patterns.Although accumulating evidences have showed circRNA plays an important role in neural development and neuropsychiatric diseases,there is lttle knowledge between circRNA and methamphetamine(MA).Using primary neuron culture,we identified the differential circRNAs with MA treatment through RNA sequencing.After RT-PCR analysis,circTLk1 expression was significant reduced.Moreover,we produced MA addicted mouse models with conditioned place preference test.The different brain area including hippocampus,prefrontal cortex,ventral tegmental area(VTA)and nucleus accubens were isolated and determined circTLk1's expression.