MiR-590-5p suppresses renal fibrosis via targeting YAP

摘要

Persistent activation of Y esassociated protein(YAP)is profoundly implicated in facilitating transformation of myofibroblasts.However,its roles in the pathogenesis of renal fibrosis remain largely unexplored.In the present study,an upregulation of YAP was detected by qRT-PCR,Western blotting,and immunohistochemistry both in the kidney tissues of folic acid(F A)-induced chronic kidney disease(CKD)mice,and in SV40MES 13 cells stimulated with TGF-β1.The microRNAs predicted to target YAP were explored by transfection of miR-590-5p mimics or inhibitors and analyzed by luciferase reporter assay.The reporter indicated that miR 590-5p directly targeted the YAP 3-untranslated region.