C16 peptide and angiopoietin-1 protect against LPS-induced BV-2 microglial cell inflammation

摘要

Pathological alterations in the brain can cause microglial activation(MA).Thus,inhibiting MA could provide a new approach for treating neurodegenerative disorders.To investigate the effect of C16 peptide and angiopoietin-1(Ang1)on inflammation following MA,we stimulated microglial BV-2 cells with lipopolysaccharide(LPS)and used dexmedetomidine(DEX)as a positive control.Specific inhibitors of Tie2,avβ3 and a5β1 integrins,and PI3K/Akt were applied to investigate the neuron-protective and anti-inflammatory effects and signaling pathway of C16+Angl treatment in the LPS-induced BV-2 cells.Our results showed that C16+Ang1 treatment reduced the microglia M1 phenotype but promoted the microglia M2 phenotype.