生后不同发育阶段大鼠卵巢内诱导型一氧化氮合酶和血管内皮生长因子的表达

摘要

目的:观察卵巢内诱导型一氧化氮合酶(iNOS)和血管内皮生长因子(VEGF)的表达,并探讨其作用.方法:用免疫组织化学技术和图像分析系统,检测了95只生后不同发育阶段大鼠卵巢内iNOS和VEGF的表达.结果:0d大鼠卵巢的卵母细胞中,iNOS和VEGF反应均呈弱阳性.4～7 d大鼠,卵母细胞和卵泡细胞中的iNOS显色,均为强阳性；VEGF显色,在卵母细胞中,原始卵泡的反应弱,初级卵泡的反应强,在卵泡细胞中则为阴性或弱阳性.14 d后,iNOS和VEGF反应在正常的卵母细胞中均呈强阳性,在退化的卵母细胞中非常弱；卵泡细胞的iNOS显色,在原始卵泡始终呈强阳性,在生长卵泡,30 d前反应强,30d后变得非常弱,在闭锁卵泡增强；VEGF显色,在所有卵泡细胞都呈较强的阳性.240 d后卵巢内间质成分和闭锁卵泡增加,其iNOS与VEGF显色均为强阳性.结论:大鼠卵巢不同发育时期,卵泡不同发育阶段,其iNOS和VEGF的表达模式有所不同,提示iNOS和VEGF间可能存在某种协同作用,共同调节卵巢的发育和功能.%Objective: The expression of iNOS and VEGF in the ovary was examined to study their role in regulating ovarian development and functions. Methods: Ovaries from 95 rats in different postnatal days were obtained to detect the expression of iNOS and VEGF by immunohistochemical technology and image analysis system. Results: Immunoreaction for iNOS and VEGF was weak positive in oocytes of rat ovaries from day 0. In rat ovaries from day 4 to 7, oocytes and follicular cells were stained strong positive for iNOS, while the positive immunostaining for VEGF was weak in oocytes of primordial follicles and strong in those of primary follicles, and negative or weak positive in follicular cells. In rat ovaries, After day 14, both of iNOS and VEGF immunoreactivities were intense in health oocytes and very weak in degenerative oocytes. Positive immunostaining for iNOS was strong all the time in follicular cells of primordial follicles. Follicular cells in growing follicles showed strong positive immunostaining for iNOS from younger rat than day 30, but became very weak from day 30. Follicular cells in atretic follicles exhibited an increase in iNOS expression comparied with those in health growing follicles. VEGF immunoreactivity was intense in all the follicular cells. Interstitium and atretic follicles increased in the ovaries from day 240, and showed strong positive staining for iNOS and VEGF. Conclusion : Expression patterns of iNOS and VEGF in rat ovaries vary with ovarian development stages and follicle types, indicating that iNOS and VEGF may work in coordination with modulating development and functions of the ovary.