白细胞介素8在着床前小鼠子宫及胚卵的表达

摘要

目的:观察着床前小鼠子宫及胚卵白细胞介素8(IL-8)的表达.方法:免疫组织化学显色及图像分析技术,对IL-8蛋白在妊娠1～4 d小鼠子宫及胚卵的表达进行定位及半定量分析；用RT-PCR技术检测妊娠1～4 d小鼠子宫及胚卵IL-8mRNA的表达情况.结果:与未孕小鼠相比,妊娠各天小鼠子宫中IL-8蛋白和mRNA表达明显升高,于妊娠4d表达最强.IL-8蛋白和mRNA均在妊娠2 d(Ⅱ细胞期)的胚卵表达最弱,妊娠4d(胚泡期)的胚卵表达最强.结论:妊娠1～4 d小鼠子宫及胚卵持续表达IL-8蛋白和mRNA,尤其是在着床前表达量升高,提示它们可能参与小鼠胚泡的着床过程.%Objective:To study the expression of interleukin-8 (IL-8) in the mouse uterus and embryo, during preimplanta-tioa Methods: The mRNA and protein expressions of IL-18 in uterus and embryo of mouse from pregnant day 1 to day 4 were detected by RT-PCR and immunohistochemistry, respectively. Results: Compared to non-pregnant mice, the mRNA and protein expressions of IL-18 in uterus of pregnant mice were significant up-regulated, and up to peak at pregnant day 4. Additionally, expression of IL-18 protein and mRNA was weak on day 2 (2-cell stage), and strongest on day 4 (blasto-cyst stage). Conclusion: IL-8 protein and IL-8 mRNA are expressed persistently in mouse uterus and embryo on day 1-4 of pregnancy, especially increase in preimplantatioa It suggests that IL-8 could participate in the process of blastocyst implantation in mouse.