N-硝基-L-精氨酸甲酯对大鼠后肢动脉生成过程中eNOS、Ki67及CD11b表达的影响

摘要

目的:探讨大鼠后肢动脉生成过程中eNOS、Ki67和CD11b的表达及N-硝基-L-精氨酸甲酯(L-NAME)对eNOS、Ki67、CD11b表达的影响.方法:18只健康SD大鼠,雌雄性各半,随机分为假手术组、模型组及L-NAME组.模型组采用大鼠股动脉结扎诱导动脉生成动物模型；L-NAME组在建立动物模型的基础上,腹腔注射L-NAME干预；假手术组除不结扎股动脉外,其他步骤与模型组相同.采用免疫荧光组织化学显色法检测血管eNOS、Ki67、CD11b等蛋白的表达.结果:eNOS主要表达于血管内皮细胞,Ki67、CD11b可表达于血管壁各层,模型组eNOS、Ki67、CD11b免疫荧光强度较假手术组强,L-NAME组eNOS、Ki67、CD11b免疫荧光强度较模型组弱.结论:在大鼠后肢动脉生成过程中,eNOS、Ki67和CD11b蛋白在血管大量表达,L-NAME可下调eNOS的表达并抑制血管增殖及巨噬细胞的浸润.%Objective:To investigate the expression of eNOS, Ki67 and CDllb, the effect of L-NAME on their expression during collateral vessels arteriogenesis in rat's hindlimb. Methods: A total of eighteen healthy SD rats were randomly divided into sham group, model group and N(omega)-nitro-L-arginine methyl ester (L-NAME) group. Femoral artery ligature was carried out in the rats of the model group and L-NAME group to induce collateral vessels arteriogenesis. Moreover, the rats in the L-NAME group were administered L-NAME intraperitoneally after operation for 7 days. One week later, the rats were sacrificed, and the expression of eNOS, Ki67 and CDllb protein was detected by immunofluorescence staining. Results: Immunohistochemically, eNOS was mainly expressed in the vascular endothelial cells, and Ki67 and CDllb were expressed in all layers of the vessel wall in the model group. The immunofluorescence intensity of eNOS, Ki67 and CDllb positive staining in the vessel of the model group were obviously increased as compared with the sham group, and the immunofluorescence intensity of eNOS, Ki67 and CDllb positive staining in the vessel of the L-NAME group was obviously decreased as compared with the model group. Conclusion: eNOS was expressed in the vascular endothelial cells. L-NAME can downregulate eNOS expression and inhibit arteriogenesis and macrophage infiltration during arteriogenesis in rat's hindlimb.