大鼠视网膜发育过程中细胞增殖与凋亡的形态学

摘要

目的：：观察视网膜发育过程中增殖细胞核抗原(PCNA)的表达变化及细胞凋亡状态。方法：14~20 d 胎鼠(E14-20 d)、生后0~15 d 幼鼠(P0-15)及成年鼠(P36),取出眼球组织,免疫组织化学显色及原位末端标记(TUNEL)观察 PCNA的表达和细胞凋亡变化。结果：发育早期 E14~P7,可观察到有较强 PCNA 阳性表达,P9时 PCNA 阳性细胞数目明显减少,P15开始未见明显 PCNA 阳性表达。E18可观察到凋亡细胞,随着发育的进行,凋亡细胞逐渐增多,P7时凋亡细胞数目最多,之后又逐渐减少,成熟的视网膜组织内未见明显凋亡细胞。结论：在视网膜神经细胞发育过程中,细胞的增殖和凋亡呈现了有序的动态变化,正是这种有序的增殖和凋亡的平衡才使视网膜最终发育成了正常的组织结构及功能。%Obj ective :To observe the change of proliferating cell nuclear antigen (PCNA)expression and cell apoptosis during the retina development of rats.Methods :Rats from embryonic days 14-20 (E14-20),postnatal days 0-1 5 (P0-1 5 )and adult (P36)were used.The eyeballs were removed and embedded in paraffin for immunohistochemical staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).Results :PCNA was firstly expressed in large quantities in rat retina from E14 to P7,decreased obviously since P9,and could not be observed since P1 5 .Apoptotic cells appeared in small quantities since E18.And then apoptotic cells increased dramatically with the retina development,and reached the peak at P7.As maturation continued,these cells decreased gradually.Apoptotic cells could not be seen in the mature retina.Conclusion:Orderly and dynamic changes of cell proliferation and apoptosis were detected during the retina development,which was essential for the development of normal structure and function of the retina.