水杨基荧光酮荧光法测定钴(Ⅱ)-过氧化氢体系产生 的羟自由基

摘要

本实验发现Co2＋与H2O2反应生成羟自由基的产率比Fenton试剂的高100倍以上。采用水杨基荧光酮(salicylfluorone,SAF)-Co(II)-H2O2荧光法测定羟自由基的新体系，激发波长和发射波长为510nm和500nm，测定体系在反应前后的荧光变化，可间接测定羟自由基产生量。此法不需昂贵的仪器设备，方法灵敏，操作简单易行。清除率实验及ESR波谱法检测证明了该方法的准确可靠，对于医用筛选抗羟自由基药物及抗羟自由基机理研究等方面具有应用价值。%It was found that the yield of hydroxyl radicals produ ced inCo(II)-H2O2 system was 100 times more than that produced in Fenton reaction. A fluorimetry method for the determination of hydroxyl radicals was developed based on salicylfluorone (SAF)-Co(II)-H2O2 system. The excitation and emission wavelengths were chosen at 500 nm and 510 nm. With measurement of fluorescence intensity, the content of hydroxyl radicals in the solution was successfully determined. The results of clearing experiment and ESR showed that the method was effective and reliable. This sensitive method can be applied to sieve the hydroxyl radical scavenger.