二硫化钼量子点荧光传感器检测盐酸多西环素的研究

摘要

通过水热法一步合成了二硫化钼(MoS2)量子点. MoS2量子点具有良好的荧光性能, 基于盐酸多西环素(DOX)对MoS2量子点荧光猝灭的特性,建立了基于MoS2量子点荧光传感器测定DOX含量的方法.研究结果表明,DOX对 MoS2量子点荧光的猝灭机理为静态猝灭.此荧光传感器测定 DOX 的线性范围为0. 86~55. 40 μg/mL,检出限为0. 023 μg/mL(S/N=3).利用此方法对盐酸多西环素片剂和加标牛奶中的DOX含量进行测定,RSD＜5% (n=3),测定结果与高效液相色谱法(HPLC)相比,相对误差＜±5% (n=3).本方法快速、简便,可用于实际样品中盐酸多西环素的测定.%Molybdenum disulfide ( MoS2) quantum dots ( QDs) were synthesized using one-step hydrothermal method with an acceptable fluorescence property. Based on the quenching effect between doxycycline hyclate ( DOX) and MoS2QDs, a fluorescence method for detection of DOX was established. The interaction between MoS2QDs and DOX was discussed with a final proposal of the static quenching mechanism. A good linear correlation for detection of DOX using fluorescenct MoS2QDs was obtained in the concentration range of 0. 86-55. 40 μg/mL, with a detection limit of 0. 023 μg/mL (S/N=3). The fabricated sensor was applied to the detection of DOX in real samples with RSDs of less than 5% . The relative error of determination results of MoS2QDs method compared with HPLC was from -4. 5% to 0. 8% and -4. 6% to 2. 8% for DOX tablet and DOX spiked milk, respectively. The fluorescence detection of DOX in real sample using MoS2QDs was simple, rapid and reliable.