设计合成了一种亲水性温敏聚合物荧光探针,用于检测农产品中的Al3+.通过可逆加成-断裂链转移(RAFT)聚合合成此聚合物荧光探针(L64-BTPA-SHMA),用1H NMR对其进行表征,并通过荧光光谱对聚合物探针的识别性能进行了研究.结果表明,此聚合物荧光探针检测Al3+的荧光响应信号受温度影响显著,温度越高,荧光强度越低.聚合物荧光探针在25℃和pH 7.4的缓冲溶液中对Al3+有良好的选择性识别作用,且基本不受其它金属离子的影响.建立了基于此聚合物探针对Al3+的荧光检测方法,响应信号与Al3+浓度在2.0~18.0 μmol/L范围内呈线性关系,相关系数为0.9977,检出限为1.43 μmol/L.此聚合物荧光探针可应用于检测农产品中Al3+残留,具有良好的实际应用价值.%A temperature-responsive hydrophilic block copolymer fluorescence sensor based on selective analysis of Al3+was designed and synthesized. The polymer fluorescent probe (L64-BTPA-SHMA) was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization and characterized by 1 H NMR,and the recognition performance of the polymer probe was studied by fluorescence spectroscopy. It was found that the fluorescence response of Al3+detected by this polymer fluorescence probe was significantly affected by temperature. The higher the temperature, the lower the fluorescence intensity was observed. The results showed that 0.1 g/L polymer fluorescent probe had a good selectivity to Al3+in buffer solution at 25℃and pH 7.4,and was not affected by other metal ions. A fluorescence detection method based on this polymer probe for Al3+was developed. In this method,the fluorescence intensity was linear with Al3+concentration in the range of 2. 0 -18. 0 μmol/L, with a correlation coefficient of 0. 9977 and a detection limit of 1.43 μmol/L. The fluorescence response between the polymer fluorescence chemosensor and Al3+could be altered by controlling the temperature change. The successful application of the polymer fluorescence probe for detecting Al3+residues in agricultural products has practical application value.
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