人血管内皮细胞生长因子D在口腔鳞癌中的表达及意义

摘要

目的 探讨人血管内皮细胞生长因子D(VEGF-D)在口腔鳞癌组织中的表达及其与口腔鳞癌临床病理特征之间的关系.方法 2006年10月-2009年10月贵阳医学院附属医院口腔颌面外科住院口腔鳞癌患者手术切除标本60例,癌旁口腔组织标本32例,同时选取正常口腔组织12例.采用免疫组织化学染色法(SABC法)检测口腔鳞癌组织、癌旁口腔组织和正常口腔组织中VEGF-D的表达,同时分析VEGF-D与患者临床病理特征的关系.采用SPSS 12.0统计软件进行统计学分析,计数资料采用χ2检验.结果 VEGF-D在口腔鳞癌组织、癌旁口腔组织及正常口腔组织中的阳性表达率分别是43.3%(26/60)、84.3%(27/32)、8.3%(1/12).癌旁口腔组织中VEGF-D的阳性表达率较口腔鳞癌组织和正常口腔组织均明显升高,差异有统计学意义(χ2值分别为14.395和18.645,P＜0.05);口腔鳞癌组织中VEGF-D的阳性表达率较正常口腔组织明显升高,差异有统计学意义(P=0.025).TNM分期Ⅰ/Ⅱ期和Ⅲ/Ⅳ期口腔鳞癌组织中VEGF-D阳性表达率比较,差异有统计学意义(χ2=5.279,P＜0.05);有淋巴结转移和无淋巴结转移口腔鳞癌组织中VEGF-D阳性表达率比较,差异有统计学意义(χ2=6.007,P＜0.05).结论 VEGF-D在口腔鳞癌组织及癌旁口腔组织中高表达,癌旁口腔组织中的表达率最高,提示其与肿瘤的侵袭性生长有关.VEGF-D在口腔鳞癌中的表达与淋巴结转移密切相关,VEGF-D可促使口腔鳞癌发生淋巴结转移.%Objective To investigate the expression of vascular endothelial growth factor D (VEGF-D) in oral squamous cell carcinoma and its relationship with clinical pathological characteristics of oral squamous cell carcinoma (OSCC). Methods 60 surgical samples of OSCC patients admitted to the Department of Oral Medicine of the Affiliated Hospital of Guiyang Medical College from October 2006 to October 2009, 32 samples of paraneoplastic tissues and 12 samples of normal oral tissues were involved into the study. Immunohistochemical staining (SABC) was used to detect the expression of VEGF-D in oral carcinoma tissues, paraneoplastic tissues and normal oral tissues, and the results were analyzed combined with the patients' clinico-pathologic indexes. SPSS 12.0 statistical software was used to make statistics analysis and enumeration data was tested by rnX . Results The positive expression rates of VEGF-D in OSCC tissues, oral paraneoplastic tissues and normal oral tissues were 43. 3% (26/60), 84. 3% (27/32) and 8. 3% (1/12) . The expression rate of VEGF-D in paraneoplastic tissues was significantly increased compared with that in OSCC tissues and normal oral tissues (X2 was 14. 395 and 18. 645 respectively, P<0. 05). The expression of VEGF-D in OSCC tissues was significantly increased compared with that in normal oral tissues (P = 0. 025). The VEGF-D protein positive rate between TNM Ⅰ/ Ⅱ stage and TNM Ⅲ/Ⅳ stage showed statistically significant difference (X2 = 5. 279, P<0. 05) . The VEGF-D protein positive rate between OSCC tissues with lymph node metastasis and OSCC tissues without lymph node metastasis showed statistically significant difference (X2 = 6. 007, P<0. 05) . Conclusion VEGF-D has high expression in OSCC tissues and paraneoplastic tissues, especially higher in the latter one, indicating that it may relate to invasive growth of the tumor. The expression of VEGF-D is closely related to lymph node metastasis and VEGF-D can promote lymph node metastasis.