Objective To explore the relationship of serumβ-arrestin 2 and free fatty acid( FFA)changes and in-sulin resistance(IR)in patients newly diagnosed as type 2 diabetes mellitus(T2DM). Methods From April to December 2012,60 newly diagnosed T2DM patients were enroll as T2DM group,30 healthy subjects as normal control( NC)group from the First Affiliated Hospital of Jiamusi University. The general data( age,height,weight,BMI)were collected,the levels of fasting blood glucose(FPG),TG,TC,FINS,β-arrestin 2,FFA determine,insulin resistance index(HOMA-IR),in-sulin sensitivity index( ISI),insulinβ-cell function index( HOMA-β)calculated. Univatiate Spearman rank correlation a-nalysis and multiple regression analysis were used to analyze the relationship between β-arrestin 2,FFA and IR. ResUlts There was no significant difference in gender,age,AST,ALT between groups NC,T2DM(P >0. 05). BMI,TG,TC, FPG,FINS,HOMA-IR,HbA1c,FFA were higher in T2DM group than in NC group(P <0. 05),and ISI,HOMA-β,β-arrestin 2 lower in T2DM group than in NC group(P<0. 05). By univariate correlation analysis,β-arrestin 2 was nega-tively correlated with BMI,FPG,TG,FINS,HOMA-IR,HbA1c,FFA(r= -0. 280,-0. 328,-0. 307,-0. 338,-0. 468,-0. 225,-0. 623,P<0. 05),positively with ISI(r=0. 439,P<0. 05);FFA was positively with BMI,FPG, TG,TC,HbA1c,FINS,HOMA-IR(r=0. 339,0. 469,0. 535,0. 277,0. 437,0. 693,0. 752,P<0. 05),negatively with ISI(r= -0. 729,P<0. 01). Multivariate regression analysis showed that FFA was an independent factor influencing IR,β-arrestin 2(standardized partial regression coefficient =0. 07,-83. 20,P<0. 05). ConclUsion β-arrestin 2 plays an important role in IR formation in T2DM patients. FFA is an independent influencing factor of IR,β-arrestin 2. Increased FFA involved in IR formation by reducingβ-arrestin 2 level,which provides a theoretical basis for FFA leading molecular mechanism of IR.%目的：探讨新诊断2型糖尿病（T2DM）患者血清β抑制蛋白（β-arrestin）2与游离脂肪酸（FFA）水平的变化及二者与胰岛素抵抗（ IR）的关系。方法选取2012年4-12月佳木斯大学附属第一医院内分泌科初诊的T2DM患者60例作为T2DM组，同时选取该院同期进行健康体检正常者30例作为正常对照（ NC）组。收集两组一般资料〔年龄、身高、体质量，计算体质指数（ BMI）〕；测定空腹血糖（ FPG）、血脂指标〔三酰甘油（ TG）、总胆固醇（TC）〕、空腹胰岛素（FINS）、血清β-arrestin 2和FFA水平，并计算胰岛素抵抗指数（HOMA-IR）、胰岛素敏感性指数（ ISI ）、胰岛素β细胞功能指数（ HOMA-β）。采用单因素Spearman秩相关分析和多元逐步回归分析β-arrestin 2、FFA与IR的关系。结果 NC组与T2DM组患者的性别构成、年龄、门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）比较，差异无统计学意义（P >0.05）。T2DM 组的 BMI、TG、TC、FPG、FINS、HOMA-IR、糖化血红蛋白（HbA1c）、FFA均高于NC组（P<0.05）；T2DM组的ISI、HOMA-β、β-arrestin 2均低于NC组（P<0.05）。单因素相关性分析显示，β-arrestin 2与BMI、FPG、TG、FINS、HOMA-IR、HbA1c、FFA均呈负相关（ r=-0.280、-0.328、-0.307、-0.338、-0.468、-0.225、-0.623，P<0.05），与ISI呈正相关（ r=0.439，P<0.05）；FFA 与 BMI、FPG、TG、TC、HbA1c、FINS、HOMA-IR均呈正相关（ r =0.339、0.469、0.535、0.277、0.437、0.693、0.752，P<0.05），与ISI呈负相关（r=-0.729，P<0.01）。多元逐步回归分析显示，FFA为HOMA-IR、β-arrestin 2的独立影响因素（标准化偏回归系数为：0.07、-83.20，P<0.05）。结论β-arrestin 2在T2DM患者体内对IR的形成发挥着重要作用，FFA是IR、β-arrestin 2的独立影响因素；T2DM患者升高的FFA可能是通过下调β-arrestin 2水平进而增强IR，为阐明FFA导致IR的分子机制提供了一定的理论基础。
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