福建地区汉族人群骨保护素基因位点多态性与急性冠脉综合征的相关性研究

摘要

目的 探讨福建地区汉族人群骨保护素(OPG)基因209G/A、245T/G、1181G/C位点基因多态性与急性冠脉综合征(ACS)及其病变程度的相关性.方法 采用前瞻性研究方法,选取2010年7月至2011年9月252例福建地区无血缘关系的汉族人为研究对象,纳入的184例ACS患者再根据冠状动脉(冠脉)造影结果分为单支病变组(60例)、双支病变组(68例)、多支病变组(54例),其中2例造影冠脉无狭窄；以68例建康体检者为对照.取静脉血提取DNA,采用Sanger测序法检测各组OPG基因209G/A、245T/G、1181G/C位点多态性.分析ACS组与对照组之间以及ACS不同病变程度组之间209G/A、245T/G、1181G/C各基因型和等位基因的分布频率以及基因多态性与ACS病变程度的关系.结果 ACS组与对照组OPG 209G/A、245T/G位点基因型及等位基因分布均无明显差异；而1181G/C位点基因型分布[GG型:92(50.0％)比43(63.2％),CC型:26(14.1％)比0,GC型:66(35.9％)比25 (36.8％),x2=11.240,P=0.004]、等位基因分布[等位基因G:250(67.9％)比111(81.6％),等位基因C:118(32.1％)比25(18.4％),x2=9.148,P=0.002]差异均有统计学意义.进一步分析发现,ACS单支病变组、双支病变组及多支病变组209G/A、245T/G及1181G/C位点分布比较均无明显差异.结论 福建地区汉族人群OPG 209G/A、245T/G位点多态性与ACS无明显相关性；1181G/C位点基因多态性与ACS发病之间存在相关性,但与其病变程度无关.%Objective To explore the association of osteoprotegerin (OPG) gene polymorphisms 209G/A,245T/G and 1181G/C in the Han population of Fujian province with acute coronary syndrome (ACS) and its severity.Methods A prospective study was conducted.Two hundred and fifty-two cases without sibship of Fujian Han people was enrolled from July 2010 to September 2011,in which 68 healthy subjects as controls.One hundred and eighty-four patients with ACS were divided into three groups,60 had one-vessel,68 had two-vessel,and 54 had three-vessel lesion,2 had not been found stegnosis.DNA was extracted from venous blood,different groups of OPG gene polymorphism was identified by Sanger Method.OPG 209G/A,245T/G and 1181G/C genotype frequencies and allelic frequencies were analyzed between ACS subjects and normal subjects and among the three groups of ACS,and the relationship between gene polymorphisms and ACS severity.Results No significant differences were observed in the genotype and allelic frequencies of OPG gene 209G/A,245T/G between the ACS and control groups.Significant differences were observed in the genotype and allelic frequencies of OPG gene 1181G/C between the ACS and control groups [1181G/C GG:92 (50.0％) vs.43 (63.2％),CC:26 (14.1％) vs.0,GC:66 (35.9％) vs.25 (36.8％),x2=11.240,P=0.004; allelic gene G:250 (67.9％) vs.111 (81.6％),allelic gene C:118 (32.1％) vs.25 (18.4％),x2=9.148,P=0.002].No significant differences were observed in the genotype and allelic frequencies of OPG gene 209G/A,245T/G and 1181G/C between those groups.Conclusions There were no significant difference in frequencies OPG genotype and allele in polymorphisms 209G/A,245T/C between patients with ACS and control group in Han population of Fujian province.There were difference in frequencies OPG genotype and allele in polymorphisms 1181G/C between patients with ACS and control group,but it was not differently distributed among patients with single-,double-,or triple-vessel lesion.