Objective To investigate the expression of 9 genes in gastric carcinoma and determine the correlation between their combination mode and 5-year survival. Methods Immunohistochemistry (IHC) method and tissue microarray technique were used to detect the expression of the 9 genes in the first group of gastric carcinoma tissues. Then, the survival related genes were screened and their expression in the second group of tissues were detected, and the correlation between their combination mode and 5-year survival was analyzed. Results IHC showed that positive expression rates of ECFR, HER-2, ANXA1 , p-mTOR, CRB2, mTOR, p53, VEGF and PCNA genes were 19. 0% , 28. 6% , 35. 5% , 46. 5% , 48. 0% , 50. 8% , 55.1 % , 59. 4% and 89. 3% .respectively. Multivariate Cox regression analysis revealed that p-mTOR, VECF and ANXA1 were independent predictive factors. Most of the expression of p-mTOR/VEGF/ANXAl was combined with the mode of+ / +/ - and -/-/ + . And survival of the gastric carcinoma patients with different combination mode of these genes' expression had significant difference. Conclusion The genotype combination mode of p-mTOR, VECF and ANXA1 could predict the prognosis and 5-year survival of gastric carcinoma in China.%目的 检测HER-2、EGFR、VEGF等9个基因在胃癌组织样本中的表达,并探讨其表达组合方式与胃癌患者5年生存率的关系.方法用免疫组化方法检测第一组胃癌组织样本的组织芯片中EGFR、HER-2、ANXA1、p-mTOR、GRB2、mTOR、p53、VEGF和PCNA共9个基因的表达情况,进行初筛,选出与胃癌患者预后密切相关的基因,然后在第二组胃癌组织样本中进行验证,分析其表达组合方式与胃癌患者5年生存率的关系.结果 免疫组化显示,EGFR、HER-2、ANXA1、pmTOR、GRB2、mTOR、p53、VEGF和PCNA基因在胃癌中的阳性表达率分别为19.0%、28.6%、35.5%、46.5%、48.0%、50.8%、55.1%、59.4%和89.3%.Cox多因素回归分析显示,p-mTOR、VEGF、ANXA1均为影响胃癌患者预后的独立因素.p-mTOR、VEGF、ANXA1基因表达以+/+/-和-/-/+组合方式占样本中的多数,且这两种组合患者的预后存在显著差异.结论p-mTOR、VEGF和ANXA13个指标的优化组合能够较为准确地判断胃癌患者的预后及5年生存率.
展开▼
