Objective To observe expression difference of associated protein such as CD34, FAPα and α⁃SMA in activated fibroblasts among tissues of transplantation tumor in rat model, tissues adjacent to tumor and normal gastric tissues and to evaluate the role of fibroblast activated state in tumor growth. Methods Sixteen rats were randomly divided into model group( n=12) and normal control group(n=4). Walker⁃256 cell lines were taken as the origin of rats model. Rat model was established by OB glue adhesive method. All rats raised at the same conditions. After 16 days, the tumor tissue, tissue adjacent to tumor and normal gastric tissue were removed. Expression of CD34, FAPα and α⁃SMA in these tissues was observed by immunohistochemical technique, Western blotting and RT⁃PCR. Results Compared with normal control group,expression of CD34 in tumor group and tissue adjacent to tumor group was significant lower( P<0�05) . But expression of FAPαandα⁃SMA in these groups was higher than control group ( P<0�05) . There were different expression between tumor tissue and tissue adjacent to tumor in FAPαandα⁃SMA(P<0�05).Conclusion The different ex⁃pression of CD34, FAPαandα⁃SMA in tumor tissue, tissue adjacent to tumor and normal gastric tissue indicated that there is correla⁃tion between fibroblasts activation and tumor development.%目的：观察间质纤维母细胞CD34、成纤维细胞激活蛋白α（ FAPα）、α⁃平滑肌肌动蛋白（α⁃SMA）在大鼠胃移植瘤与正常胃组织中的表达差异，探讨间质纤维母细胞活化状态在肿瘤发生、发展中的作用。方法采用walker256细胞株建立大鼠胃移植瘤模型（ n＝12），以正常大鼠为对照（ n＝4），均在同等SPF级条件下饲养。16天后处死大鼠，取肿瘤组织、瘤周组织及正常饲养大鼠胃组织，免疫组化染色、Western blotting、RT⁃PCR法检测各组组织中CD34、FAPα、α⁃SMA的表达。结果与正常对照组相比，肿瘤组织及瘤周组织中CD34蛋白及基因水平的表达均低于正常对照组，差异具有统计学意义（ P＜0�05），而FAPα和α⁃SMA表达则明显高于正常对照组（ P＜0�05）；肿瘤组织与瘤周组织之间FAPα和α⁃SMA表达差异亦具有统计学意义（ P＜0�05）。结论 CD34、FAPα与α⁃SMA在肿瘤组织、瘤周组织及正常组织中的表达存在差异，提示间质纤维母细胞活化状态与肿瘤发生发展存在相关性。
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