CD1a与CD83标记肿瘤浸润性树突状细胞在乳腺癌中的表达及相关性研究

摘要

Objective To explore the expression of CD1a and CD83 marked tumor infiltrating dendritic cells(TIDC) in breast tissues and its correlation with prognosis. Methods Eighty⁃five cases of breast cancer tissues were collected and CD1a, CD83 expressions of breast cancer tissues were detected by immunochemical method. The relationship between their expression and clinico⁃pathological features and prognosis were analyzed. Results There were 77 cases with CD1a positive expression in 85 cases of breast cancer tissues, and most TIDC expressing CD1a were distributed in tumor tissues, and less expressed in tumor stroma and surrounding tissues;there were 44 cases with CD83 positive expression in 85 cases of breast cancer tissues, and TIDC expressing CD83 were ob⁃served in surrounding and interstitial tissues of tumors, with scattering distribution. There were 35 cases with CD1a and CD83 double positive expression. CD1a, CD83 double positive expression had no correlation with lymph node metastasis, progestrogen receptor( PR) and neutrophil⁃lymphocyte ratio(NLR)(P>0�05), but correlated with estrogen receptor(ER), HER⁃2 and TNM staging(P<0�05). The median disease⁃free survival( DFS) of patients with both CD1a, CD83 double positive expression and non⁃double positive expres⁃sion were not reached. Multivariate analysis indicated that non⁃double positive expression of CD1a, CD83 was an independent factor af⁃fecting prognosis of patients (HR=2�216,95%CI:1�682⁃3�431;P<0�05). Conclusion The expression of characteristic markers CD83 and CD1a of TIDC in breast cancer tissues are related with prognosis, and may be associated with immune escape mechanism of tumors, so they can be the reference indicators to detect the prognosis of breast cancer.%目的：探讨CD1a和CD83标记的肿瘤浸润性树突状细胞（ TIDC）在乳腺组织中的表达及其与临床病理特征和预后的关系。方法采用免疫组织化学法检测85例乳腺癌组织中CD1a、CD83的表达情况，探讨其与乳腺癌临床病理特征及预后的关系。结果在85例乳腺癌组织中，77例CD1a阳性，大多数表达CD1a的TIDC分布在肿瘤组织中，肿瘤间质及周边组织中表达较少；44例CD83阳性，表达CD83的TIDC多分布在肿瘤周边及间质组织，呈散点状分布。 CD1a、CD83均为阳性表达（双阳性）35例。 CD1a、CD83双阳性表达与淋巴结转移、孕激素受体（PR）、中性粒细胞⁃淋巴细胞比值（NLR）无关（P＞0�05），而与雌激素受体（ ER）、表皮生长因子受体（ HER⁃2）、TNM分期有关（ P＜0�05）。 CD1a、CD83双阳性表达和非双阳性表达患者的中位无病生存期（ DFS）均未达。 Cox多因素回归分析显示， CD1a、CD83非双阳性表达是影响DFS的独立因素（ HR＝2�216，95％CI：1�682～3�431；P＜0�05）。结论乳腺癌组织中TIDC的特征性标记物CD83、CD1a表达是影响DFS的独立因素，这可能与肿瘤免疫逃逸机制有关，可以作为乳腺癌预后的参考指标。