抗人心肌线粒体抗体在病毒性心肌炎和扩张型心肌病中的检测及其意义

摘要

Objectives:The present study in vestigated the presence and significance of serum antibody against human myocardial mitochondria in patients with virus myocarditis (VMC) and dilated cardiomyopathy (DCM).rn　　Methods:The human myocardial mitochondtia were used as antigen to detect serum autoantibody by immuno-dot blot in 29 VMC patients,24 DCM patients,33 patients with other cardiac (OCD) and 20 healthy blood donor (HBD).The antigen molecular weight was detected by Western blot.rn　　Results:①Positive autoantibody against human myocardial mitochordria was found in 41.4% in VMC and 41.7% in DCM patients,which were much higher than 6.1% in OCD or 0% in HBD patients.②Cardiac troponin T was elevated in 43.8% of autoantibody-positive VMC and DCM patients,which was much higher than 12.0% in autoantibody-negative VMC and DCM patients (p＜0.05).③The antigen molecular weight of human myocardial mitochondria was 30KD.rn　　Conclusions:①The presence of autoantibody against supports that concept that is responsible for the development of VMC and DCM.Autoantibody is one of the factors that give rise to cardiac injury.The antibody detection may serve as a diagnostic index for VMC and DCM.②The specific antigen is probably human myocardial adenine nucleotide translocator.%目的：检测病毒性心肌炎(VMC)和扩张型心肌病(DCM)患者血清中是否有抗人心肌线粒体抗体存在及其意义。rn 方法：以人心肌线粒体作抗原，应用免疫点印迹在29例VMC(VMC组)、24例DCM(DCM组)、33例其它心脏病(OCD组)及20例健康献血者(HBD组)进行抗人心肌线粒体抗体检测，用免疫印迹方法检测其抗原分子量。rn 结果：VMC组(12例，41.4%)DCM组(10例，41.7%)抗人心肌线粒体抗体阳性明显高于OCD组(2例，6.1%)及HBD(0%)；②抗体阳性的VMC和DCM患者中有43.8%的心肌肌钙蛋白T升高，抗体阴性者为12.0%(P＜0.05)有显著性差异；③人心肌线粒体特异性抗原的分子量为30KD。rn 结论：①在VMC和DCM患者血清中存在抗人心肌线粒体抗体，说明部分VMC及DCM与自身免疫有关，该抗体是引起心肌损伤的因素之一，对该抗体的检测可作为VMC和DCM的辅助诊断手段之一；②该抗体的特异性抗原可能为人心肌线粒体腺苷酸转位酶。