In this article, in vitro adsorption of aflatoxin B1 (AFB1) onto different adsorbents was characterized and the result was verified by comparing the growth performance and serum protein levels of broilers exposed to aflatoxin-contamination feed. Main components of adsorbents selected were yeast cell extracts (Product A), HSCAS (Product B), and a mixture of yeast product and HSCAS (Product C), respectively. A total of 240 broilers were assigned to eight treatments, and the effects of three types of adsorbents on growth performance and serum protein levels were evaluated. Results indicated that Product B had the highest in vitro affinity for AFB1, followed by Product C and Product A. Product B bound 97.69% of the AFB1 in solution in 10 min, and it remained over 96.03% in 60 min at pH 8.0. The B-AFB1 complex was much stronger than the other two complexes in vitro condition (P < 0.05). Feed intake (FI) and average daily gain (ADG) decreased (P < 0.05) and feed gain ratio increased (P < 0.05) in the treatment fed aflatoxin-contaminated feed versus treatment on the basal feed. Serum total protein (TP), albumin (ALB), and globulin (GLOB) levels were significantly decreased (P < 0.05). Product B (0.15%) increased growth performance and improved serum protein levels, Product A and Product C were not as effective as Product B. Three adsorbents tested here had sufficient potential to AFB1 in some extents and Product B could bind AFB1 more effectively than Product A and Product C. These results indicated that Product B could alleviate some of the AFB1 toxic effect in broilers.
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