CD4+CD25+调节性T淋巴细胞在急性脑梗死患者外周血中表达的初步研究

摘要

目的 初步探讨CD4+CD25+ 调节性T淋巴细胞在急性脑梗死患者外周血中的表达情况.方法 采用流式细胞术分别检测取98例急性脑梗死患者和50名健康体检者外周血中CD4+CD25+T细胞所占比例,并用双抗体夹心法检测各组血清TGF-β、IL-10和IL-2.结果 急性脑梗死患者外周血中CD4+CD25+T细胞水平显著高于对照组(P<0.01),血清中TGF-β、IL-10和IL-2显著高于对照组,差异有统计学意义(P<0.01).结论 CD4+CD25+T细胞在急性脑梗死患者外周血中表达显著升高,血清中的相关细胞因子参与诱导CD4+CD25+T细胞的生成,提示CD4+CD25+T细胞可能参与了急性脑梗死的发生、发展.%Objective To investigate the proportion of CD4+CD25+Tregs in the peripheral blood of the patients suffering from acut cerebralin farction(ACI). Methods The proportion of CD4+CD25+Tregs in the peripheral blood of two groups of people (the patients with ACI and the healthy donors) was evaluated by flow cytometry. The serum TGF-βand IL-10 levels were detected by ELISA. Results The percentage of CD4+CD25+T cells in the patients with ACI was significantly higher than that of the healthy donors(P<0.01). The serum level of TGF-βand IL-10 in experimental group were statistically higher than those in control group(P<0.01). Conclusion The proportion of CD4+CD25+T cells in the peripheral blood of the patients with ACI significantly higher than that of heaIthy donors. Moreover cytokines associated with Tregs can contribute to the production of CD4+CD25+T cells in the patients with ACI, which indicated that CD4+CD25+T cells might play certain role in the pathogenesis and progress in ACI.