目的：探讨脑源性神经营养因子(BDNF)基因功能性多态(rs6265)与散发性阿尔茨海默病(SAD)发病的相关性。方法选取58例散发性阿尔茨海默患者(SAD组)与52例健康老年人(对照组),用聚合酶链反应-限制性片段长度多态性技术,对BDNF基因rs6265,进行基因型检测,同时利用酶联免疫吸附技术对两组患者的血清BDNF水平进行检测。结果在<60岁的人群中,等位基因(χ2=6.0595, P=0.013)及基因型(χ2=6.0826,P=0.0478)在两组人群中的分布差异有统计学意义,并且SAD的AA基因型患者的血清BDNF水平最低[(14.32±4.21)ng/ml, F=7.2545, P=0.0016]。结论 BDNF基因功能性多态rs6265与SAD发病相关,并且影响其血清BDNF的表达。%Objective To explore the relationship between functional polymorphism of BDNF(brain-derived neurotrophic factor) rs6265 and sporadic Alzheimer’s disease(SAD). Methods Selecting 58 cases of SAD(SAD group) and 52 health old person(control group) as research subjects. Then the genotyped was detected via polymerase chain reaction-restriction fragment length polymorphism and serum levels of BDNF detected by Enzyme-linked Immunosorbent Assay in all cases. Results There was significantly difference in the distributions of alleles(χ2=6.0595,P=0.013) and genotypes(χ2=6.0826,P=0.0478) between the case of SAD group and control group ,and the serum levels of BDNF were lowest for case of AA genotypes in SAD[(14.32±4.21) ng/ml, F=7.2545, P=0.0016]. Conclusion The functional polymorphism of BDNF gene(rs6265) correlated with pathogenesis of SAD and affects the expression of BDNF in serum.
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