因宁片对甲亢性肝损害模型大鼠肝脏细胞色素P450基因表达的影响

摘要

目的:研究因宁片对甲亢性肝损害模型大鼠肝脏细胞色素P450(CYP450)基因表达的影响.方法:采用L-甲状腺激素钠灌胃以复制甲亢性肝损害大鼠模型.实验分为6组,即空白对照、模型、甲亢灵和因宁片高、中、低剂量(2.4、1.2、0.6g·kg-1)组.通过实时荧光定量聚合酶联反应(PCR)方法检测因宁片对肝组织内CYP1 A2、CYP2E1和CYP3A2基因表达的影响.结果:与模型组比较,因宁片高、中剂量组大鼠肝脏CYP2E1的表达显著减弱(P＜0.05),CYP1A2和CYP3A2的表达无明显改变(P＞0.05).结论:因宁片能下调甲亢大鼠肝脏CYP2E1基因的表达,这可能是因宁片治疗甲亢性肝损害的分子作用机制之一.%OBJECTIVE: To study the effects of Yinning tablet on gene expression of cytochrome P450(CYP450) in liver tissue of rats with hyperthyroidism liver damage. METHODS: Rats were perfused with levothyroxine sodium intragastrically to establish the rat model of hyperthyroidism liver damage. The model rats were divided into 6 groups, I.e. Blank control group, model group, Ji-akangning group and Yinning tablet high-dose, medium-dose and low-dose groups (2.4, 1.2,0.6 g·kg-1). The expressions of CYP1A2,CYP2E1 and CYP3A2 in liver tissue were detected by real-time quantitative PCR. RESULTS: Compared with model group, the expressions of CYP2E1 was decreased significantly(P0.05). CONCLUSION: Yinning tablet may down-regulate the expression of CYP2E1 in liver tissue of rats with hyperthyroidism liver damage, which may be one of the molecular action mechanisms that Yinning tablet prevent hyperthyroidism liver damage.