HPLC法测定补肾防喘薄膜衣片中淫羊藿苷的含量

摘要

OBJECTIVE:To establish an HPLC method for the determination of icariin in Bushen Fangchuan Film-coaling Tablets.METHODS:The chromatographic column was DiamonsilTM C18 with acetonitrile-water(30∶ 70,V/V) as mobile phase at the flow rate of 1.0 ml/min.Column temperature was 30 ℃.The detection wavelength was set at 270 nm and injection volume was 10 μl.RESULTS:The good linearity was obtained in the range of 0.049 9 μg-0.998 0 μg for ieariin(r=0.999 8).RSDs of precision,stability and reproducibility tests were lower than and equal to 0.83％.The average recovery was 100.35％ (RSD=0.69％,n=9).CONCLUSIONS:The method is simple with strong specifity and good reproducibility,can control the quality of this preparation.%目的:建立测定补肾防喘薄膜衣片中淫羊藿苷含量的方法.方法:采用高效液相色谱法.色谱柱为DiamonsilTM C18柱,流动相为乙腈-水(30∶70,V/V),流速为1.0 ml/min,柱温为30℃,检测波长为270 nm,进样量为10μl.结果:淫羊藿苷进样量在0.049 9～0.998 0 μg范围内与峰面积积分值呈良好的线性关系(r=0.999 8)；精密度、稳定性、重复性试验的RSD均≤0.83％；平均加样回收率为100.35％,RSD=0.69％ (n=9).结论:该方法操作简单、专属性强、重复性好,能够有效控制该制剂的质量.