OBJECTIVE:To investigate inhibitory effects of matrine on the proliferation of human bladder cancer BIU-87 cells and its mechanism. METHODS:The cell viability was detected by MTT assay and inhibitory rate of cell proliferation was calculat-ed after human bladder cancer BIU-87 cells were treated with 0(negative control),0.5,1.0,2.0 and 4.0 mg/ml matrine for 24,48 and 72 h,respectively. After treated with 0 (negative control),0.5,1.0,2.0 and 4.0 mg/ml matrine for 48 h,the cell cycle and apoptotic rate were detected by flow cytometry;the expression of Survivin,Caspase-3 and Caspase-7 protein were detected by Western blot assay. RESULTS:Compared with negative control,the proliferation of BIU-87 cells were significantly inhibited after incubated with 1.0-4.0 mg/ml matrine for 24,48 and 72 h(P<0.05 or P<0.01),and inhibitory rate of cell proliferation increased in concentration and time-dependant manner;after treated for 48 h,the percentage of G0/G1 phase cells and apoptotic rate in-creased,while the percentage of cells at S phase and G2/M phase were decreased (P<0.05 or P<0.01);the expression of Cas-pase-3 and Caspase-7 protein increased,while the expression of survivin protein decreased after incubated with 0.5-4.0 mg/ml ma-trine for 48 h. CONCLUSIONS:Matrine can inhibit the proliferation of human bladder cancer BIU-87 cells,block the cell cycle and induce apoptosis;its mechanism may be related to the expression regulation of Survivin,Caspase-3 and Caspase-7.%目的：研究苦参碱对人膀胱癌BIU-87细胞增殖的抑制作用及其机制。方法：以0（阴性对照）、0.5、1.0、2.0、4.0 mg/ml苦参碱分别作用于人膀胱癌BIU-87细胞24、48、72 h后，MTT法检测细胞活性并计算细胞增殖抑制率；以0（阴性对照）、0.5、1.0、2.0、4.0 mg/ml苦参碱作用于细胞48 h后，流式细胞术检测细胞周期和凋亡率，Western blot法检测细胞中生存素、天冬氨酸半胱氨酸蛋白酶（Caspase）-3及Caspase-7蛋白的表达。结果：与阴性对照比较，1.0～4.0 mg/ml苦参碱作用24、48、72 h后均对BIU-87细胞增殖有显著的抑制作用（P＜0.05或P＜0.01），增殖抑制率呈浓度和时间依赖性升高；作用48 h后，G0/G1期细胞比例升高、S期及G2/M期细胞比例降低、凋亡率升高（P＜0.05或P＜0.01）。0.5～4.0 mg/ml苦参碱作用48 h后，细胞中生存素蛋白表达减弱、Caspase-3及Caspase-7蛋白表达增强（P＜0.05或P＜0.01）。结论：苦参碱可抑制人膀胱癌BIU-87细胞的增殖、阻滞细胞周期、诱导细胞凋亡，其机制可能与调控细胞中生存素及Caspase-3、Caspase-7的表达有关。
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