促红细胞生成素抑制正常及缺铁性贫血大鼠铁调素的表达

摘要

Objective: To investigate the effect of erythropoietin ( EPO ) on the serum hepcidin expression in iron deficiency anemia ( IDA ) rats. Method: IDA rat model was established by repeated bloodletting and low-iron diet feeding. The hepcidin expression in normal rats with or without EPO intervention and IDA rats with or without EPO intervention was respectively detected and compared. Result: After the intra-peritoneal injection of EPO, hepcidin expression and C/EBPα levels decreased in the IDA group. EPO also significantly inhibited hepcidin expression in the normal rats( P＜0. 05 ). Conclusion: Hepcidin plays an important role in the pathogene-sis of IDA. Both in normal and IDA rats, EPO can decrease hepcidin expression levels by declining C/EBPα to hinder the activation of the hepcidin promoter.%目的:探讨促红细胞生成素(EPO)在缺铁性贫血(IDA)大鼠模型中对铁调素(hepcidin)表达的影响.方法:通过反复放血和饲喂低铁饲料,建立IDA大鼠动物模型,比较药物干预组(EPO)与非干预组及正常对照组与正常给药组(EPO)大鼠血清hepcidin表达的差异.Western blot检测肝脏内信号因子CCAAT/增强子结合蛋白α(C/EBPα)含量.结果:IDA模型组腹腔注射EPO后,血清hepcidin和肝脏C/EBPα含量显著降低.EPO对正常大鼠hepcidin表达也有显著抑制作用(P<0.05).结论:hepcidin可能参与了IDA的发生,在正常大鼠和IDA大鼠中,EPO能通过下调C/EBPα阻碍其激活hepcidin启动子最终抑制hepcidin的表达.