HPLC法测定喉咽清口服液中齐墩果酸-28-O-β-D-吡喃葡萄糖苷含量

摘要

Objective:To establish a method for the content determination of oleanolic acid-28-O-β-D-glucopyranoside in Houyanqing oral liquids.Method:The analysis was performed on a Hibar C18 (250 mm × 4.6 mm,5 μm)column with the mobile phase of acetonitrile-0.4％ phosphoric acid (34∶ 66).The flow rate was 1.0 ml · min-1 and the detection wavelength was 203 nm.Result:The linear range of oleanolic acid-28-O-β-D-glucopyranoside was 0.146-4.848 μg (r =1.000 0),and the average recovery was 98.86％ (RSD =1.62％,n =6).Conclusion:The method is simple and accurate,and can provide the reference for the establishment of the quality standards.%目的:建立喉咽清口服液中齐墩果酸-28-O-β-D-吡喃葡萄糖苷的含量测定方法.方法:色谱柱为Hibar C18 (250 mm×4,6 mm,5μm),乙腈-0.4％磷酸(34∶66)为流动相;流速为1.0 ml·min-1;检测波长为203 nm.结果:齐墩果酸-28-O-β-D-吡喃葡萄糖苷在0.146～4.848 μg(r=1.000 0)范围内线性关系良好,平均回收率为98.86％,RSD为1.62％(n=6).结论:所建立的方法简便,准确,为该制剂质量标准的建立提供一定的参考.