曼地亚红豆杉与南方红豆杉不同部位中紫杉醇含量比较研究

摘要

Objective:To compare the taxol content in different parts of Taxus madia and Taxus chinensis var. mairei to provide reference for the future researches. Methods:The active ingredients were extracted by a mixed solvent (50% acetone and 50% ethyl acetate) , the target compound taxol was separated by a neutral Al2 O3 solid phase column, and then the taxol content was analyzed and compared among the different parts of Taxus madia and Taxus chinensis var. mairei by HPLC. Results:Taxol in velamen of Taxus ma-dia had the highest content among all the samples, which was 0. 0439% and 88 times as high as that in the bark of Taxus chinensis var. mairei. Conclusion:Among all the samples of Taxus madia and Taxus chinensis var. mairei, velamen of Taxus madia has the highest taxol content.%目的：：比较曼地亚红豆杉和南方红豆杉不同部位抗癌活性成分紫杉醇的含量，为红豆杉资源开发利用提供依据。方法：利用丙酮和乙酸乙酯混合溶剂提取有效成分，用中性Al2 O3吸附柱色谱法粗提紫杉醇，用高效液相色谱法测定紫杉醇含量。结果：曼地亚红豆杉各部位紫杉醇含量均高于南方红豆杉，根皮中紫杉醇含量最高，达0.0439%，约是南方红豆杉茎皮中紫杉醇含量的88倍。结论：曼地亚红豆杉根皮、茎皮、叶、幼枝、果实中均含有紫杉醇，其含量高于南方红豆杉茎皮中紫杉醇含量，可以对曼地亚红豆杉进行综合开发利用。