HPLC法同时测定不同产地荔枝草中齐墩果酸和熊果酸含量

摘要

Objective:To develop an HPLC method for the simultaneous determination of oleanic acid and ursolic acid in Salvia plebeia R. Br. from different origins. Methods:The column was Agilent-C18 (250 mm × 4. 6 mm,5μm) with the temperature of 25℃, the mobile phase was methanol-water-glacial acetic acid-triethylamine (90 ∶10 ∶0. 03∶0. 06) at the flow rate of 1. 0 ml·min-1 ,and the detection wavelength was 210 nm. Results: The calibration curves were linear within the range of 7. 50-75. 00 μg · ml-1 for oleanic acid(r=0. 999 7)and 5. 00-50. 00 μg · ml-1 for ursolic acid(r=0. 999 7). The average recovery of oleanic acid and ursolic acid was 100. 15%(RSD=1. 14%,n=6)and 99. 40%(RSD=1. 67%,n=6), respectively. Conclusion: The method is simple and accurate, which is suitable for the determination of oleanic acid and ursolic acid in Salvia plebeia R. Br. from different origins.%目的：：建立HPLC法同时测定不同产地荔枝草中齐墩果酸和熊果酸的含量。方法：采用Agilent-C18色谱柱(250 mm ×4.6 mm,5μm),流动相为甲醇-水-冰醋酸-三乙胺(90∶10∶0.03∶0.06),检测波长：210 nm,流速：1.0 ml·min-1,柱温：25℃。结果：齐墩果酸在7.50~75.00μg· ml-1、熊果酸在5.00~50.00μg · ml-1浓度范围内线性关系良好,r均为0.9997。齐墩果酸和熊果酸的平均加样回收率分别为100.15%(RSD=1.14%,n=6)、99.40%(RSD=1.67%,n=6)。结论：该方法简便、准确,适用于荔枝草中齐墩果酸和熊果酸的含量测定。