HPLC法测定复合维生素制剂中全反式维生素A棕榈酸酯的含量

摘要

目的:建立复合维生素制剂中全反式维生素A棕榈酸酯的含量测定方法.方法:采用HPLC法,色谱柱:Apollo Silica(250 mm×4.6 mm,5μm);流动相:正己烷-异丙醇(999.5:0.5);流速:1.0 ml·min-1;柱温:30℃;检测波长:325 nm;进样量:100μl.结果:全反式维生素A棕榈酸酯的线性范围为0.2260~0.6780 IU·ml-1(相当于0.1243~0.3729μg·ml-1,r=0.9999),平均回收率为99.86%,RSD为0.64%(n=9).结论:本法简便、准确、灵敏度高、重复性好、通用性强,可用于复合维生素制剂中全反式维生素A棕榈酸酯的含量测定.%Objective:To determine the content of all-trans-retinyl palmitate in multivitamin preparations. Methods: HPLC was used and the chromatographic column was Apollo Silica (250 mm × 4. 6 mm, 5 μm). The mobile phase consisted of hexane-isopro-panol (999. 5:0. 5) and the column temperature was 30 ℃. The detection wavelength was 325 nm and the flow rate was 1. 0 ml· min-1 . The injection volume was 100 μl. Results:The calibration curve of all-trans-retinyl palmitate was linear within the concentra-tion range of 0. 2260-0. 6780 IU·ml-1(0. 1243-0. 3729 μg·ml-1, r=0. 9999). The average recovery was 99. 86%, and RSD was 0. 64%(n=9). Conclusion:The method is simple,accurate,sensitive,reproducible and universal, which can be used for the con-tent determination of all-trans-retinyl palmitate in multivitamin preparations.