川东獐牙菜中苦龙苷含量测定研究

摘要

目的 采用高效液相色谱法测定川东獐牙菜中苦龙苷的含量.方法 以Hypersil Cis柱(150 mm×4.6 mm,5 μm)为色谱柱,甲醇-水(50:50)为流动相,检测波长227 nm,流速1 mL/min,柱温为室温.结果 苦龙苷进样量在0.33～3.96 μg范围内与峰面积线性关系良好,r=0.999 6(n=6):平均回收率为99.11%,RSD=1.48%(n=9).结论 高效液相色谱法用于测定川东獐牙菜中苦龙苷的含量,方法简便快速、结果准确.%Objective To determine the content of amarogenti in Swertia davidi Franch.by HPLC.Methods The analysis was carried out on Shimadzu C18 column(150 mm ×4.6mm,5 μm).The mobile phase was MeOH-H2O(50∶ 50).The wavelength was 227 nm and the flowrate was 1 mL/min.The temperature was room temperature.Results The linear range of amarogenti is 0.33-3.96 μg, r =0.999 6( n = 6).The average recovery rate was 99.11％, RSD was 1.48％ (n = 9).Conclusion The HPLC method was simple,fast and had accurate results.